Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.39 extracted from

  • Koppisch, A.T.; Khosla, C.
    Structure-based mutagenesis of the malonyl-CoA:acyl carrier protein transacylase from Streptomyces coelicolor (2003), Biochemistry, 42, 11057-11064.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Streptomyces coelicolor

Protein Variants

Protein Variants Comment Organism
A197D site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptomyces coelicolor
F200A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
F200S site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
F200Y site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptomyces coelicolor
G198A site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptomyces coelicolor
G198Y site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
M126I site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
M137L site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
Q9A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
R122A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
R122K site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
T57V site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Streptomyces coelicolor
V98Q site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
malonyl-CoA pH 6.8, recombinant mutant M137L Streptomyces coelicolor
0.019
-
malonyl-CoA pH 6.8, recombinant wild-type enzyme Streptomyces coelicolor
0.023
-
malonyl-CoA pH 6.8, recombinant mutant M126I Streptomyces coelicolor
0.032
-
malonyl-CoA pH 6.8, recombinant mutant V98Q Streptomyces coelicolor
0.034
-
malonyl-CoA pH 6.8, recombinant mutant A197D Streptomyces coelicolor
0.05
-
malonyl-CoA pH 6.8, recombinant mutant F200S Streptomyces coelicolor
0.06
-
malonyl-CoA pH 6.8, recombinant mutants G198Y and G198A Streptomyces coelicolor
0.075
-
[acyl-carrier protein] pH 6.8, recombinant mutant F200Y Streptomyces coelicolor
0.083
-
malonyl-CoA pH 6.8, recombinant mutant F200A Streptomyces coelicolor
0.095
-
malonyl-CoA pH 6.8, recombinant mutant F200Y Streptomyces coelicolor
0.1
-
[acyl-carrier protein] pH 6.8, recombinant mutants M137L and F200S Streptomyces coelicolor
0.12
-
[acyl-carrier protein] pH 6.8, recombinant wild-type enzyme and mutant V98Q Streptomyces coelicolor
0.13
-
[acyl-carrier protein] pH 6.8, recombinant mutants A197D and G198Y Streptomyces coelicolor
0.15
-
malonyl-CoA pH 6.8, recombinant mutant R122A Streptomyces coelicolor
0.15
-
[acyl-carrier protein] pH 6.8, recombinant mutants M126I and G198A Streptomyces coelicolor
0.2
-
malonyl-CoA pH 6.8, recombinant mutant Q9A Streptomyces coelicolor
0.24
-
[acyl-carrier protein] pH 6.8, recombinant mutant F200A Streptomyces coelicolor
0.4
-
malonyl-CoA pH 6.8, recombinant mutant T57V Streptomyces coelicolor
0.57
-
[acyl-carrier protein] pH 6.8, recombinant mutant T57V Streptomyces coelicolor
0.7
-
malonyl-CoA pH 6.8, recombinant mutant R122K Streptomyces coelicolor
0.92
-
[acyl-carrier protein] pH 6.8, recombinant mutant Q9A Streptomyces coelicolor
1.1
-
[acyl-carrier protein] pH 6.8, recombinant mutant R122A Streptomyces coelicolor
3.5
-
[acyl-carrier protein] pH 6.8, recombinant mutant R122K Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + [acyl-carrier protein] Streptomyces coelicolor enzyme provides acyl-ACP thioesters for the biosynthesis of aromatic polyketides, overview, and thus is the primary gatekeeper of substrate specificity in type II polyketide synthase CoA + malonyl-[acyl-carrier protein]
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) Streptomyces coelicolor

Reaction

Reaction Comment Organism Reaction ID
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] putative active site structure and involved catalytic residues Streptomyces coelicolor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + [acyl-carrier protein] enzyme provides acyl-ACP thioesters for the biosynthesis of aromatic polyketides, overview, and thus is the primary gatekeeper of substrate specificity in type II polyketide synthase Streptomyces coelicolor CoA + malonyl-[acyl-carrier protein]
-
?
malonyl-CoA + [acyl-carrier protein] the active site is rigorously ordered around the acyl-thioester moiety of the acyl-CoA to facilitate rapid and efficient transacylation to an ACP Streptomyces coelicolor CoA + malonyl-[acyl-carrier protein]
-
?

Synonyms

Synonyms Comment Organism
malonyl-CoA:acyl carrier protein transacylase
-
Streptomyces coelicolor
MAT
-
Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
malonyl-CoA pH 6.8, recombinant mutant F200S Streptomyces coelicolor
0.3
-
malonyl-CoA pH 6.8, recombinant mutant M137L Streptomyces coelicolor
0.35
-
malonyl-CoA pH 6.8, recombinant mutant R122A Streptomyces coelicolor
0.4
-
malonyl-CoA pH 6.8, recombinant mutants G198Y Streptomyces coelicolor
0.7
-
malonyl-CoA pH 6.8, recombinant mutant Q9A Streptomyces coelicolor
0.94
-
malonyl-CoA pH 6.8, recombinant mutant F200A Streptomyces coelicolor
3 6 malonyl-CoA pH 6.8, recombinant mutant F200Y Streptomyces coelicolor
3
-
malonyl-CoA pH 6.8, recombinant mutant M126I Streptomyces coelicolor
11
-
malonyl-CoA pH 6.8, recombinant mutant R122K Streptomyces coelicolor
12
-
malonyl-CoA pH 6.8, recombinant mutants G198A Streptomyces coelicolor
25
-
malonyl-CoA pH 6.8, recombinant mutant T57V Streptomyces coelicolor
70
-
malonyl-CoA pH 6.8, recombinant mutant A197D Streptomyces coelicolor
95
-
malonyl-CoA pH 6.8, recombinant mutant V98Q Streptomyces coelicolor
100
-
malonyl-CoA pH 6.8, recombinant wild-type enzyme Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Streptomyces coelicolor