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Literature summary for 2.3.1.39 extracted from

  • Molnos, J.; Gardiner, R.; Dale, G.E.; Lange, R.
    A continuous coupled enzyme assay for bacterial malonyl-CoA:acyl carrier protein transacylase (FabD) (2003), Anal. Biochem., 319, 171-176.
    View publication on PubMed

Application

Application Comment Organism
diagnostics coupled enzyme assay can be used in development and optimization of small-molecule inhibitors for antibacterial therapy Escherichia coli K-12

Cloned(Commentary)

Cloned (Comment) Organism
from K-12 strain MC4100, overexpression in strain M15 Escherichia coli K-12

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
malonyl-CoA pH 6.8, 28°C, recombinant enzyme Escherichia coli K-12

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + [acyl-carrier protein] Escherichia coli K-12 key enzyme in fatty acid synthesis CoA + malonyl-[acyl-carrier protein] malonyl-[acyl-carrier protein] is the key building block for de novo fatty acid synthesis ?

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12
-
gene fabD
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from strain M15 Escherichia coli K-12

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + [acyl-carrier protein] key enzyme in fatty acid synthesis Escherichia coli K-12 CoA + malonyl-[acyl-carrier protein] malonyl-[acyl-carrier protein] is the key building block for de novo fatty acid synthesis ?
malonyl-CoA + [acyl-carrier protein] transfer to the free thiol group of the phosphopantetheine arm of the [acyl-carrier protein] Escherichia coli K-12 CoA + malonyl-[acyl-carrier protein]
-
?
additional information development of a continuous fluorometric coupled assay method in microplates by coupling of the enzyme to alpha-ketoglutarate dehydrogenase consuming CoA, overview Escherichia coli K-12 ?
-
?

Synonyms

Synonyms Comment Organism
FabD
-
Escherichia coli K-12
malonyl-CoA:acyl carrier protein transacylase
-
Escherichia coli K-12
MCAT
-
Escherichia coli K-12

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Escherichia coli K-12

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Escherichia coli K-12