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Literature summary for 2.3.1.39 extracted from

  • Zhang, L.; Joshi, A.K.; Hofmann, J.; Schweizer, E.; Smith, S.
    Cloning, expression, and characterization of the human mitochondrial beta-ketoacyl synthase. Complementation of the yeast CEM1 knock-out strain (2005), J. Biol. Chem., 280, 12422-12429.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic tree, expression as GFP-fusion protein containing the mitochondrial targeting sequence in mitochondria of HeLa cells, expression of truncated enzyme forms lacking the first 21 or 59 amino acid residues, respectively, in Escherichia coli strain BL21(DE3) as soluble enzymes, or in Spodoptera frugiperda Sf9 cells via baculovirus infection system Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion enzyme is part of the malonyl-CoA-dependent type II fatty acid synthase complex in mitochondria, and contains a mitochondrial targeting sequence Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + [acyl-carrier protein] Homo sapiens absolutely specific for the substrates, type II [acyl-carrier protein] CoA + malonyl-[acyl-carrier protein]
-
?
additional information Homo sapiens enzyme is part of the malonyl-CoA-dependent type II fatty acid synthase complex in mitochondria ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant truncated enzyme forms from Sf9 insect cells Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1
-
recombinant enzyme with recombinant ACP-A from Bacillus subtilis Homo sapiens
0.14
-
recombinant enzyme with recombinant human mitochondrial [acyl-carrier protein] lacking the first 68 amino acid residues Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + [acyl-carrier protein] absolutely specific for the substrates, type II [acyl-carrier protein] Homo sapiens CoA + malonyl-[acyl-carrier protein]
-
?
malonyl-CoA + [acyl-carrier protein] absolutely specific for the substrates, recombinant type II [acyl-carrier protein] lacking the N-terminal 68 amino acid residues from human, or recombinant ACP-A from Bacillus subtilis Homo sapiens CoA + malonyl-[acyl-carrier protein]
-
?
additional information enzyme is part of the malonyl-CoA-dependent type II fatty acid synthase complex in mitochondria Homo sapiens ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Homo sapiens