Cloned (Comment) | Organism |
---|---|
expression of five mutants in Escherichia coli | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the Km for N-acetylcysteamine is significantly higher than for an acyl-carrier-protein | Streptomyces coelicolor | |
0.0208 | - |
malonyl-CoA | wild-type enzyme | Streptomyces coelicolor | |
0.0239 | - |
malonyl-CoA | S97A mutant | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Streptomyces coelicolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + acyl-carrier protein | - |
Escherichia coli | CoA + malonyl-[acyl-carrier protein] | - |
? | |
malonyl-CoA + acyl-carrier protein | - |
Streptomyces coelicolor | CoA + malonyl-[acyl-carrier protein] | - |
? | |
malonyl-CoA + N-acetylcysteamine | - |
Escherichia coli | CoA + N-acetyl-S-malonylcysteamine | - |
? | |
malonyl-CoA + N-acetylcysteamine | in the presence of high N-acetylcysteamine concentrations the forward reaction can be driven to completion | Streptomyces coelicolor | CoA + N-acetyl-S-malonylcysteamine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
25 | - |
assay at | Streptomyces coelicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000933 | - |
N-acetylcysteamine | S97A mutant | Streptomyces coelicolor | |
0.00683 | - |
N-acetylcysteamine | N-acetylcysteamine as acceptor thiol, wild-type enzyme | Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Streptomyces coelicolor |