Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.39 extracted from

  • Szafranska, A.E.; Hitchman, T.S.; Cox, R.J.; Crosby, J.; Simpson, T.J.
    Kinetic and mechanistic analysis of the malonyl CoA:ACP transacylase from Streptomyces coelicolor indicates a single catalytically competent serine nucleophile at the active site (2002), Biochemistry, 41, 1421-1427.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a hexahistidine-tagged fusion protein Streptomyces coelicolor

General Stability

General Stability Organism
enzyme labile during chromatography Streptomyces coelicolor

Inhibitors

Inhibitors Comment Organism Structure
phenylmethanesulfonyl fluoride
-
Escherichia coli
phenylmethanesulfonyl fluoride
-
Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
malonyl-CoA hexahistidine-tagged fusion protein, reaction with Streptomyces coelicolor acyl-carrier-protein Streptomyces coelicolor
0.073
-
acyl-carrier protein hexahistidine-tagged fusion protein, reaction with Streptomyces coelicolor acyl-carrier-protein Streptomyces coelicolor
0.25
-
malonyl-CoA reaction with E. coli acyl-carrier-protein Escherichia coli
0.327
-
malonyl-CoA hexahistidine-tagged fusion protein, reaction with E. coli acyl-carrier-protein Streptomyces coelicolor
0.351
-
acyl-carrier protein reaction with E. coli acyl-carrier-protein Escherichia coli
0.776
-
acyl-carrier protein reaction with E. coli acyl-carrier-protein Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34110
-
electrospray mass spectrometry, hexahistidine-tagged fusion protein Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Streptomyces coelicolor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the hexahistidine-tagged fusion protein, using Ni(II) affinity chromatography, column chromatography on Q-Sepharose, phenyl Superose and Mono-Q Streptomyces coelicolor

Reaction

Reaction Comment Organism Reaction ID
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] mechanism, kinetic studies Streptomyces coelicolor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli
additional information
-
hexahistidine-tagged fusion protein Streptomyces coelicolor
780
-
hexahistidine-tagged fusion protein, at pH 6.5 and 30°C Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + acyl-carrier protein
-
Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
?
malonyl-CoA + acyl-carrier protein
-
Streptomyces coelicolor CoA + malonyl-[acyl-carrier protein]
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli
30
-
assay at Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
420
-
malonyl CoA hexahistidine-tagged fusion protein, reaction with E. coli acyl-carrier-protein Streptomyces coelicolor
450
-
malonyl CoA hexahistidine-tagged fusion protein, reaction with Streptomyces coelicolor acyl-carrier-protein Streptomyces coelicolor
1580
-
malonyl CoA reaction with E. coli acyl-carrier-protein Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Escherichia coli
6.5
-
assay at Streptomyces coelicolor