Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activation | Glycine max | |
malonate | at 0.1 mM, MCT1 is doubled in activity and MCT2 is 30-40% than control | Glycine max |
General Stability | Organism |
---|---|
freezing leads to 20% loss of isozyme MCT1-activity | Glycine max |
glycerol, 20% v/v, stabilizes isozyme MCT1 | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetyl-CoA | inhibition of both isozymes at 0.02 mM and at 0.1 mM | Glycine max | |
CoA | not inhibitory | Glycine max | |
diethyldicarbonate | inhibits MCT1 but not MCT2 at 1 mM | Glycine max | |
malonyl-CoA | inhibition of MCT1 at 0.01 mM and inhibition of both isozymes at 0.02 mM and 0.04 mM; inhibits both MCT1 and MCT2 | Glycine max | |
methylmalonyl-CoA | inhibition of MCT1 at 0.1 mM | Glycine max | |
additional information | pH-dependent inhibition of malonyl-enzyme intermediate formation by N-ethylmaleimide | Escherichia coli | |
N-ethylmaleimide | - |
Escherichia coli | |
N-ethylmaleimide | 1 mM, isozyme MCT2: 40% loss of activity of isozyme MCT2, no effect on isozyme MCT1, both, MCT1 and MCT2, are inactivated at 10 mM | Glycine max | |
phenylmethanesulfonyl fluoride | not inhibitory | Glycine max | |
propionyl-CoA | inhibition of both isozymes at 0.1 mM | Glycine max | |
reduced CoA | inhibition of both isozymes at 0.1 mM | Glycine max | |
succinyl-CoA | inhibition of MCT2 at 0.02 mM and inhibition of both isozymes at 0.1 mM | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0094 | - |
malonyl CoA | - |
Glycine max | |
0.015 | - |
malonyl CoA | - |
Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
HPLC-gel permeation chromatography, 2 isozymic forms HPLC analysis | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Glycine max | - |
soy bean, strain AMSOY71 | - |
Purification (Comment) | Organism |
---|---|
of 2 isozymes MCT1 and MCT2, using salt fractionation, ion-exchange chromatography, and chromatofocusing | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | isozymes 1 and 2 | Glycine max | - |
seed | isozyme 1 is predominant | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.32 | - |
isozyme MCT1 | Glycine max |
1 | - |
isozyme MCT2 | Glycine max |
Storage Stability | Organism |
---|---|
-80°C, stable in the presence of glycerol | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + acyl-carrier protein | both isozymes may not be completely specific for malonyl-CoA as the substrate | Glycine max | CoA + malonyl-[acyl-carrier protein] | - |
? | |
malonyl-CoA + acyl-carrier protein | acetyl-CoA cannot replace malonyl-CoA | Escherichia coli | CoA + malonyl-[acyl-carrier protein] | - |
? | |
malonyl-CoA + acyl-carrier protein | acetyl-CoA cannot replace malonyl-CoA | Glycine max | CoA + malonyl-[acyl-carrier protein] | - |
? | |
malonyl-CoA + acyl-carrier protein | via stable covalent malonyl-enzyme intermediate | Escherichia coli | CoA + malonyl-[acyl-carrier protein] | - |
? | |
malonyl-CoA + acyl-carrier protein | via stable covalent malonyl-enzyme intermediate | Glycine max | CoA + malonyl-[acyl-carrier protein] | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Glycine max |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Escherichia coli |
50 | - |
isozyme MCT1: 50% loss of activity after 5 min and 60% loss of activity after 10 min, isozyme MCT2: 30% increase of activity after 10 min | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Glycine max |
8.5 | - |
- |
Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | maximal activity | Glycine max |