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Literature summary for 2.3.1.39 extracted from

  • Guerra, D.J.; Ohlrogge, J.B.
    Partial purification and characterization of two forms of malonyl-coenzyme A:acyl carrier protein transacylase from soybean leaf tissue (1986), Arch. Biochem. Biophys., 246, 274-285.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activation Glycine max
malonate at 0.1 mM, MCT1 is doubled in activity and MCT2 is 30-40% than control Glycine max

General Stability

General Stability Organism
freezing leads to 20% loss of isozyme MCT1-activity Glycine max
glycerol, 20% v/v, stabilizes isozyme MCT1 Glycine max

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA inhibition of both isozymes at 0.02 mM and at 0.1 mM Glycine max
CoA not inhibitory Glycine max
diethyldicarbonate inhibits MCT1 but not MCT2 at 1 mM Glycine max
malonyl-CoA inhibition of MCT1 at 0.01 mM and inhibition of both isozymes at 0.02 mM and 0.04 mM; inhibits both MCT1 and MCT2 Glycine max
methylmalonyl-CoA inhibition of MCT1 at 0.1 mM Glycine max
additional information pH-dependent inhibition of malonyl-enzyme intermediate formation by N-ethylmaleimide Escherichia coli
N-ethylmaleimide
-
Escherichia coli
N-ethylmaleimide 1 mM, isozyme MCT2: 40% loss of activity of isozyme MCT2, no effect on isozyme MCT1, both, MCT1 and MCT2, are inactivated at 10 mM Glycine max
phenylmethanesulfonyl fluoride not inhibitory Glycine max
propionyl-CoA inhibition of both isozymes at 0.1 mM Glycine max
reduced CoA inhibition of both isozymes at 0.1 mM Glycine max
succinyl-CoA inhibition of MCT2 at 0.02 mM and inhibition of both isozymes at 0.1 mM Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0094
-
malonyl CoA
-
Glycine max
0.015
-
malonyl CoA
-
Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
HPLC-gel permeation chromatography, 2 isozymic forms HPLC analysis Glycine max

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Glycine max
-
soy bean, strain AMSOY71
-

Purification (Commentary)

Purification (Comment) Organism
of 2 isozymes MCT1 and MCT2, using salt fractionation, ion-exchange chromatography, and chromatofocusing Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
leaf isozymes 1 and 2 Glycine max
-
seed isozyme 1 is predominant Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.32
-
isozyme MCT1 Glycine max
1
-
isozyme MCT2 Glycine max

Storage Stability

Storage Stability Organism
-80°C, stable in the presence of glycerol Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + acyl-carrier protein both isozymes may not be completely specific for malonyl-CoA as the substrate Glycine max CoA + malonyl-[acyl-carrier protein]
-
?
malonyl-CoA + acyl-carrier protein acetyl-CoA cannot replace malonyl-CoA Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
?
malonyl-CoA + acyl-carrier protein acetyl-CoA cannot replace malonyl-CoA Glycine max CoA + malonyl-[acyl-carrier protein]
-
?
malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
?
malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Glycine max CoA + malonyl-[acyl-carrier protein]
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
-
Escherichia coli
50
-
isozyme MCT1: 50% loss of activity after 5 min and 60% loss of activity after 10 min, isozyme MCT2: 30% increase of activity after 10 min Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Glycine max
8.5
-
-
Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5 maximal activity Glycine max