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Literature summary for 2.3.1.39 extracted from

  • Stapleton, S.R.; Jaworski, J.G.
    Characterization and purification of malonyl-coenzyme A:[acyl-carrier protein] transacylases from spinach and Anabaena variabilis (1984), Biochim. Biophys. Acta, 794, 240-248.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
CoA kinetics Spinacia oleracea
CoA kinetics Trichormus variabilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
malonyl-CoA sequential mechanism Trichormus variabilis
0.4
-
malonyl-CoA ping-pong mechanism Trichormus variabilis
0.4
-
acyl-carrier protein sequential mechanism Spinacia oleracea
0.4
-
acyl-carrier protein sequential mechanism Trichormus variabilis
0.5
-
malonyl-CoA sequential mechanism Spinacia oleracea
0.6
-
acyl-carrier protein ping-pong mechanism Trichormus variabilis
1.3
-
acyl-carrier protein ping-pong mechanism Spinacia oleracea
1.4
-
malonyl-CoA ping-pong mechanism Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
HPLC gel filtration Spinacia oleracea
36000
-
HPLC gel filtration Trichormus variabilis

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
spinach
-
Trichormus variabilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation and DEAE-cellulose Spinacia oleracea
ammonium sulfate fractionation and DEAE-cellulose Trichormus variabilis

Reaction

Reaction Comment Organism Reaction ID
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] mechanism, kinetic studies Spinacia oleracea
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] mechanism, kinetic studies Trichormus variabilis

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0683
-
purified enzyme Spinacia oleracea
0.1541
-
purified enzyme Trichormus variabilis

Storage Stability

Storage Stability Organism
-20°C, frozen in liquid nitrogen, several months Spinacia oleracea
-20°C, frozen in liquid nitrogen, several months Trichormus variabilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Spinacia oleracea CoA + malonyl-[acyl-carrier protein]
-
?
malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Trichormus variabilis CoA + malonyl-[acyl-carrier protein]
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Spinacia oleracea
25
-
assay at Trichormus variabilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
inactivation after 20 min Spinacia oleracea
80
-
inactivation after 20 min Trichormus variabilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
higher activity in Tris buffer than in potassium phosphate buffer Spinacia oleracea
8.5
-
higher activity in Tris buffer than in potassium phosphate buffer Trichormus variabilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information
-
Spinacia oleracea
additional information
-
additional information
-
Trichormus variabilis
0.009
-
CoA with respect to acyl carrier protein Trichormus variabilis
0.015
-
CoA with respect to malonyl-CoA Trichormus variabilis
0.023
-
CoA with respect to malonyl-CoA Spinacia oleracea
0.03
-
CoA with respect to acyl carrier protein Spinacia oleracea