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Literature summary for 2.3.1.39 extracted from

  • Ruch, F.E.; Vagelos, P.R.
    Characterization of a malonyl-enzyme intermediate and identification of the malonyl binding site in malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli (1973), J. Biol. Chem., 248, 8095-8106.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information inhibition of malonyl-enzyme intermediate formation by p-chloromercuribenzoate, reversible by 2-mercaptoethanol, prevented by preincubation with malonyl-CoA; inhibition of malonyl-enzyme intermediate formation by phenylmethanesulfonyl fluoride, malonyl-CoA protects; pH-dependent inhibition of malonyl-enzyme intermediate formation by N-ethylmaleimide Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] isolation and characterization of an enzyme-intermediate Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + acyl-carrier protein acetyl-CoA cannot replace malonyl-CoA Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
?
malonyl-CoA + acyl-carrier protein participation of a malonyl-enzyme intermediate in the reaction Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.5 for the malonyl-enzyme intermediate formation. The enzyme accepts malonyl groups from either malonyl-CoA or malonyl-acyl-carrier-protein to form the enzyme-intermediate, the malonyl group of the intermediate can be transferred to both CoA and acyl-carrier-protein Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
of malonyl-enzyme intermediate Escherichia coli