General Stability | Organism |
---|---|
dithiothreitol stabilizes | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | malonyl-CoA protects; pH-dependent | Escherichia coli | |
additional information | apparently homogeneous enzyme not inhibited by sulfhydryl agents, N-ethylmaleimide and iodoacetamide, highly purified enzyme stimulated by dithiothreitol | Escherichia coli | |
N-ethylmaleimide | malonyl-CoA protects; pH-dependent inhibition of purified enzyme | Escherichia coli | |
phenylmethanesulfonyl fluoride | - |
Escherichia coli | |
sulfhydryl compounds | purified enzyme | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 35000, SDS-PAGE, alkylated: 1 * 35500, SDS-PAGE, native: 1 * 36500, SDS-PAGE | Escherichia coli |
35500 | - |
1 * 35000, SDS-PAGE, alkylated: 1 * 35500, SDS-PAGE, native: 1 * 36500, SDS-PAGE | Escherichia coli |
36500 | - |
1 * 35000, SDS-PAGE, alkylated: 1 * 35500, SDS-PAGE, native: 1 * 36500, SDS-PAGE | Escherichia coli |
36660 | - |
carboxymethylated enzyme: sedimentation equilibrium centrifugation | Escherichia coli |
37000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chromatography on DEAE-cellulose, Sephadex G-100, Sephadex G-75, DEAE-Sephadex, and preparative polyacrylamide gel electrophoresis | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] | isolation and characterization of an enzyme-intermediate | Escherichia coli | |
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] | mechanism, formation of a malonyl-enzyme intermediate | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1851 | - |
purified enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + acyl-carrier protein | - |
Escherichia coli | CoA + malonyl-[acyl-carrier protein] | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE, alkylated: 1 * 35500, SDS-PAGE, native: 1 * 36500, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | about half-maximal activity at pH 5.0, about 80% of maximal activity at pH 10.0 | Escherichia coli |