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Literature summary for 2.3.1.39 extracted from

  • Ruch, F.E.; Vagelos, P.R.
    The isolation and general properties of Escherichia coli malonyl coenzyme A-acyl carrier protein transacylase (1973), J. Biol. Chem., 248, 8086-8094.
    View publication on PubMed

General Stability

General Stability Organism
dithiothreitol stabilizes Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide malonyl-CoA protects; pH-dependent Escherichia coli
additional information apparently homogeneous enzyme not inhibited by sulfhydryl agents, N-ethylmaleimide and iodoacetamide, highly purified enzyme stimulated by dithiothreitol Escherichia coli
N-ethylmaleimide malonyl-CoA protects; pH-dependent inhibition of purified enzyme Escherichia coli
phenylmethanesulfonyl fluoride
-
Escherichia coli
sulfhydryl compounds purified enzyme Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
1 * 35000, SDS-PAGE, alkylated: 1 * 35500, SDS-PAGE, native: 1 * 36500, SDS-PAGE Escherichia coli
35500
-
1 * 35000, SDS-PAGE, alkylated: 1 * 35500, SDS-PAGE, native: 1 * 36500, SDS-PAGE Escherichia coli
36500
-
1 * 35000, SDS-PAGE, alkylated: 1 * 35500, SDS-PAGE, native: 1 * 36500, SDS-PAGE Escherichia coli
36660
-
carboxymethylated enzyme: sedimentation equilibrium centrifugation Escherichia coli
37000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
chromatography on DEAE-cellulose, Sephadex G-100, Sephadex G-75, DEAE-Sephadex, and preparative polyacrylamide gel electrophoresis Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] isolation and characterization of an enzyme-intermediate Escherichia coli
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] mechanism, formation of a malonyl-enzyme intermediate Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1851
-
purified enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + acyl-carrier protein
-
Escherichia coli CoA + malonyl-[acyl-carrier protein]
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE, alkylated: 1 * 35500, SDS-PAGE, native: 1 * 36500, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8.5
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5 10 about half-maximal activity at pH 5.0, about 80% of maximal activity at pH 10.0 Escherichia coli