Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | 59% inhibition at 0.1 mM | Escherichia coli | |
N-ethylmaleimide | 79% inhibition at 0.1 mM | Escherichia coli | |
sulfhydryl compounds | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using streptomycin sulfate, column chromatography on DEAE-cellulose, ammonium sulfate fractionation, chromatography on Sephadex G-100 I column, DEAE-Sephadex column and Sephadex G-100 II column | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.04 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 2-4 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + acyl-carrier protein | acetyl-CoA cannot replace malonyl-CoA | Escherichia coli | CoA + malonyl-[acyl-carrier protein] | - |
? | |
malonyl-CoA + acyl-carrier protein | the enzyme is very specific for the malonyl group | Escherichia coli | CoA + malonyl-[acyl-carrier protein] | - |
? | |
malonyl-CoA + pantetheine | - |
Escherichia coli | malonyl-pantetheine + CoA | - |
? | |
malonyl-pantetheine + acyl-carrier protein | - |
Escherichia coli | pantetheine + malonyl-[acyl-carrier protein] | - |
? | |
additional information | mercaptoethanol is inactive as a substitute for acyl-carrier-protein | Escherichia coli | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is quite stable to heat | Escherichia coli |
80 | - |
20% loss of activity after 20 min | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Escherichia coli |