BRENDA - Enzyme Database show
show all sequences of 2.3.1.39

The characteristics of some components of the fatty acid synthetase system in the plastids from the mesocarp of avocado (Persea americana) fruit

Caughey, I.; Kekwick, R.G.O.; Eur. J. Biochem. 123, 553-561 (1982)

Data extracted from this reference:

General Stability
General Stability
Organism
dithiothreitol stabilizes
Persea americana
EDTA stabilizes
Persea americana
glycerol, 15% v/v, stabilizes
Persea americana
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
inhibition of the enzyme by thiol-alkylating reagents
Persea americana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00134
-
pantetheine
-
Persea americana
0.00326
-
malonyl-CoA
-
Persea americana
0.042
-
acyl-carrier protein
-
Persea americana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
plastid
from the mesocarp
Persea americana
9536
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40500
-
gel filtration, elution from Sephadex G-100
Persea americana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
malonyl-CoA + acyl-carrier protein
Persea americana
the enzyme is a component of the fatty acid synthetase system
CoA + malonyl-acyl-carrier protein
-
Persea americana
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Persea americana
-
avocado
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate fractionation and column chromatography on DEAE-cellulose
Persea americana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mesocarp
mesocarp
Persea americana
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
9.59
-
-
Persea americana
Storage Stability
Storage Stability
Organism
-20°C, potassium-phosphate buffer, pH: 7.0, 1 mM dithiothreitol, 0.5 mM EDTA, 15% v/v glycerol
Persea americana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonyl-CoA + acyl-carrier protein
via stable covalent malonyl-enzyme intermediate
285678
Persea americana
CoA + malonyl-[acyl-carrier protein]
-
285678
Persea americana
?
malonyl-CoA + acyl-carrier protein
the enzyme is a component of the fatty acid synthetase system
285678
Persea americana
CoA + malonyl-acyl-carrier protein
-
285678
Persea americana
?
malonyl-CoA + pantetheine
-
285678
Persea americana
malonyl-pantetheine + CoA
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Persea americana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Persea americana
8.3
-
-
Persea americana
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.2
9.8
about half-maximal activity at pH 6.2, about 65% of maximal activity at pH 9.8
Persea americana
General Stability (protein specific)
General Stability
Organism
dithiothreitol stabilizes
Persea americana
EDTA stabilizes
Persea americana
glycerol, 15% v/v, stabilizes
Persea americana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
inhibition of the enzyme by thiol-alkylating reagents
Persea americana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00134
-
pantetheine
-
Persea americana
0.00326
-
malonyl-CoA
-
Persea americana
0.042
-
acyl-carrier protein
-
Persea americana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
plastid
from the mesocarp
Persea americana
9536
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40500
-
gel filtration, elution from Sephadex G-100
Persea americana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
malonyl-CoA + acyl-carrier protein
Persea americana
the enzyme is a component of the fatty acid synthetase system
CoA + malonyl-acyl-carrier protein
-
Persea americana
?
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate fractionation and column chromatography on DEAE-cellulose
Persea americana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mesocarp
mesocarp
Persea americana
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
9.59
-
-
Persea americana
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, potassium-phosphate buffer, pH: 7.0, 1 mM dithiothreitol, 0.5 mM EDTA, 15% v/v glycerol
Persea americana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonyl-CoA + acyl-carrier protein
via stable covalent malonyl-enzyme intermediate
285678
Persea americana
CoA + malonyl-[acyl-carrier protein]
-
285678
Persea americana
?
malonyl-CoA + acyl-carrier protein
the enzyme is a component of the fatty acid synthetase system
285678
Persea americana
CoA + malonyl-acyl-carrier protein
-
285678
Persea americana
?
malonyl-CoA + pantetheine
-
285678
Persea americana
malonyl-pantetheine + CoA
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Persea americana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Persea americana
8.3
-
-
Persea americana
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.2
9.8
about half-maximal activity at pH 6.2, about 65% of maximal activity at pH 9.8
Persea americana
Other publictions for EC 2.3.1.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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3
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1
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1
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2
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1
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-
-
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2
1
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-
-
-
1
-
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1
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1
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-
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1
1
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2
2
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1
-
-
-
-
2
1
-
-
-
-
1
-
-
1
-
1
1
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60-65
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-
-
-
-
-
-
-
-
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12
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4
4
-
-
-
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20-28
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-
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1
-
-
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1
1
1
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1
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5
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
1
-
-
-
720468
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-
1
1
-
-
-
-
-
-
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4
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701546
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20-22
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1
1
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-
-
-
-
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4
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719494
Hong
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FEBS Lett.
584
1240-1244
2010
-
-
2
2
5
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-
-
-
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4
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2
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2
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2
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2
5
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2
-
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2
-
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-
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-
701440
Arthur
Structure and malonyl CoA-ACP ...
Streptomyces coelicolor
ACS Chem. Biol.
4
625-636
2009
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1
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1
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703315
Gurvitz
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Comp. Funct. Genomics
2009
836172
2009
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1
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1
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1
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3
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705648
Misra
Catalysis and mechanism of mal ...
Escherichia coli, Plasmodium falciparum
Mol. Biosyst.
5
651-659
2009
-
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-
10
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4
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4
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2
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5
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10
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4
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2
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5
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5
5
701486
Jung
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Acta Crystallogr. Sect. F
64
1143-1145
2008
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1
1
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6
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684213
Ghadbane
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Acta Crystallogr. Sect. F
F63
831-835
2007
-
-
1
1
-
-
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6
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688366
Li
The crystal structure of MCAT ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Biol.
371
1075-1083
2007
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1
1
3
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3
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1
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4
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1
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1
1
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1
1
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1
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689905
Zhang
Malonyl-CoA: acyl carrier prot ...
Helicobacter pylori
Protein Sci.
16
1184-1192
2007
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1
1
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4
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671071
Oefner
Mapping the active site of Esc ...
Escherichia coli
Acta Crystallogr. Sect. D
D62
613-618
2006
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1
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3
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673026
Arthur
The malonyl transferase activi ...
Streptomyces coelicolor
Chem. Biol.
13
587-596
2006
-
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2
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1
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1
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676953
Huang
Purification and characterizat ...
Mycobacterium tuberculosis
Protein Expr. Purif.
45
393-399
2006
-
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1
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5
-
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1
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3
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1
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1
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1
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659451
Zhang
Cloning, expression, and chara ...
Homo sapiens
J. Biol. Chem.
280
12422-12429
2005
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6
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1
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3
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1
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1
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6
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Joshi
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4
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1
1
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5
1
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4
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1
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1
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5
1
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486880
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3
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1
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1
5
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1
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2
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1
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3
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1
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1
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1
1
5
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1
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2
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1
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486865
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1
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1
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4
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