General Stability | Organism |
---|---|
dithiothreitol stabilizes | Persea americana |
EDTA stabilizes | Persea americana |
glycerol, 15% v/v, stabilizes | Persea americana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | inhibition of the enzyme by thiol-alkylating reagents | Persea americana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00134 | - |
pantetheine | - |
Persea americana | |
0.00326 | - |
malonyl-CoA | - |
Persea americana | |
0.042 | - |
acyl-carrier protein | - |
Persea americana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | from the mesocarp | Persea americana | 9536 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40500 | - |
gel filtration, elution from Sephadex G-100 | Persea americana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + acyl-carrier protein | Persea americana | the enzyme is a component of the fatty acid synthetase system | CoA + malonyl-acyl-carrier protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Persea americana | - |
avocado | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation and column chromatography on DEAE-cellulose | Persea americana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mesocarp | mesocarp | Persea americana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.59 | - |
- |
Persea americana |
Storage Stability | Organism |
---|---|
-20°C, potassium-phosphate buffer, pH: 7.0, 1 mM dithiothreitol, 0.5 mM EDTA, 15% v/v glycerol | Persea americana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + acyl-carrier protein | via stable covalent malonyl-enzyme intermediate | Persea americana | CoA + malonyl-[acyl-carrier protein] | - |
? | |
malonyl-CoA + acyl-carrier protein | the enzyme is a component of the fatty acid synthetase system | Persea americana | CoA + malonyl-acyl-carrier protein | - |
? | |
malonyl-CoA + pantetheine | - |
Persea americana | malonyl-pantetheine + CoA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Persea americana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Persea americana |
8.3 | - |
- |
Persea americana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 9.8 | about half-maximal activity at pH 6.2, about 65% of maximal activity at pH 9.8 | Persea americana |