BRENDA - Enzyme Database show
show all sequences of 2.3.1.38

Purification of plant acetyl-CoA: acyl carrier protein transacylase

Shimakata, T.; Stumpf, P.K.; Methods Enzymol. 122, 53-59 (1986)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
arsenite
-
Spinacia oleracea
N-ethylmaleimide
-
Spinacia oleracea
PCMB
-
Spinacia oleracea
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.008
-
acetyl-CoA
-
Spinacia oleracea
0.0083
-
butyryl-CoA
-
Spinacia oleracea
0.0086
-
hexanoyl-CoA
-
Spinacia oleracea
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
gel filtration
Spinacia oleracea
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + [acyl-carrier protein]
Spinacia oleracea
rate-limiting step of fatty acid synthesis
CoA + acetyl-[acyl-carrier protein]
-
Spinacia oleracea
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Spinacia oleracea
-
var. Viroflay
-
Purification (Commentary)
Commentary
Organism
partial
Spinacia oleracea
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0056
-
partially purified enzyme
Spinacia oleracea
Storage Stability
Storage Stability
Organism
-70°C, several months
Spinacia oleracea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + [acyl-carrier protein]
-
486866
Spinacia oleracea
CoA + acetyl-[acyl-carrier protein]
-
486866
Spinacia oleracea
?
acetyl-CoA + [acyl-carrier protein]
rate-limiting step of fatty acid synthesis
486866
Spinacia oleracea
CoA + acetyl-[acyl-carrier protein]
-
486866
Spinacia oleracea
?
butyryl-CoA + [acyl-carrier protein]
35% activity of that with acetyl-CoA
486866
Spinacia oleracea
CoA + butyryl-[acyl-carrier protein]
-
-
-
?
hexanoyl-CoA + [acyl-carrier protein]
18% activity of that with acetyl-CoA
486866
Spinacia oleracea
CoA + hexanoyl-[acyl-carrier protein]
-
-
-
?
additional information
no substrate: malonyl-CoA
486866
Spinacia oleracea
?
-
-
-
-
octanoyl-CoA + [acyl-carrier protein]
poor substrate
486866
Spinacia oleracea
CoA + octanoyl-[acyl-carrier protein]
-
-
-
?
octanoyl-CoA + [acyl-carrier protein]
7% activity of that with acetyl-CoA
486866
Spinacia oleracea
CoA + octanoyl-[acyl-carrier protein]
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
38
-
assay at
Spinacia oleracea
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.1
-
-
Spinacia oleracea
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
9.1
about half-maximal activity at pH 7.5 and about 75% of maximal activity at pH 9.1
Spinacia oleracea
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
arsenite
-
Spinacia oleracea
N-ethylmaleimide
-
Spinacia oleracea
PCMB
-
Spinacia oleracea
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.008
-
acetyl-CoA
-
Spinacia oleracea
0.0083
-
butyryl-CoA
-
Spinacia oleracea
0.0086
-
hexanoyl-CoA
-
Spinacia oleracea
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
gel filtration
Spinacia oleracea
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + [acyl-carrier protein]
Spinacia oleracea
rate-limiting step of fatty acid synthesis
CoA + acetyl-[acyl-carrier protein]
-
Spinacia oleracea
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Spinacia oleracea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0056
-
partially purified enzyme
Spinacia oleracea
Storage Stability (protein specific)
Storage Stability
Organism
-70°C, several months
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + [acyl-carrier protein]
-
486866
Spinacia oleracea
CoA + acetyl-[acyl-carrier protein]
-
486866
Spinacia oleracea
?
acetyl-CoA + [acyl-carrier protein]
rate-limiting step of fatty acid synthesis
486866
Spinacia oleracea
CoA + acetyl-[acyl-carrier protein]
-
486866
Spinacia oleracea
?
butyryl-CoA + [acyl-carrier protein]
35% activity of that with acetyl-CoA
486866
Spinacia oleracea
CoA + butyryl-[acyl-carrier protein]
-
-
-
?
hexanoyl-CoA + [acyl-carrier protein]
18% activity of that with acetyl-CoA
486866
Spinacia oleracea
CoA + hexanoyl-[acyl-carrier protein]
-
-
-
?
additional information
no substrate: malonyl-CoA
486866
Spinacia oleracea
?
-
-
-
-
octanoyl-CoA + [acyl-carrier protein]
poor substrate
486866
Spinacia oleracea
CoA + octanoyl-[acyl-carrier protein]
-
-
-
?
octanoyl-CoA + [acyl-carrier protein]
7% activity of that with acetyl-CoA
486866
Spinacia oleracea
CoA + octanoyl-[acyl-carrier protein]
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
38
-
assay at
Spinacia oleracea
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.1
-
-
Spinacia oleracea
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
9.1
about half-maximal activity at pH 7.5 and about 75% of maximal activity at pH 9.1
Spinacia oleracea
Other publictions for EC 2.3.1.38
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
486945
Waters
Functional characterization of ...
Plasmodium falciparum
Mol. Biochem. Parasitol.
123
85-94
2002
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486875
Lobo
A Streptomyces collinus thiola ...
Streptomyces collinus
Biochemistry
40
11955-11964
2001
-
-
1
-
1
-
2
2
-
-
3
-
-
4
-
-
1
-
-
-
1
-
1
1
1
-
1
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
2
-
2
-
-
3
-
-
-
-
1
-
-
1
-
1
1
1
-
1
2
-
-
-
-
-
-
-
-
-
-
486874
Rangan
Fatty acid synthase dimers con ...
Rattus norvegicus
J. Biol. Chem.
273
34949-34953
1998
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486873
Rangan
Alteration of the substrate sp ...
Rattus norvegicus
J. Biol. Chem.
272
11975-11978
1997
-
-
1
-
2
-
-
3
-
-
-
-
-
3
-
-
1
1
1
-
4
-
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
1
1
-
4
-
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
486872
Gulliver
Acetoacyl-acyl carrier protein ...
Persea sp.
Plant Mol. Biol.
25
179-191
1994
-
-
-
-
-
-
2
-
2
-
1
-
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
1
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
486871
Worsham
Early catalytic steps of Eugle ...
Euglena gracilis
Biochim. Biophys. Acta
1170
62-71
1993
-
-
-
-
-
-
4
-
2
-
1
2
-
1
-
-
1
-
-
-
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
2
-
1
2
-
-
-
1
-
-
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
486868
Lowe
Purification and characterizat ...
Escherichia coli, Escherichia coli B / ATCC 11303
Biochem. J.
250
789-796
1988
-
-
-
-
-
-
8
2
-
-
2
-
-
177
-
-
1
1
-
-
1
1
2
1
1
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
8
2
2
-
-
2
-
-
-
-
1
-
-
1
1
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
486866
Shimakata
Purification of plant acetyl-C ...
Spinacia oleracea
Methods Enzymol.
122
53-59
1986
-
-
-
-
-
-
3
3
-
-
1
1
-
2
-
-
1
-
-
1
1
1
7
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
1
1
-
-
-
1
-
1
1
1
7
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
285675
Shimakata
The purification and function ...
Brassica juncea, Carthamus tinctorius, Cuphea lutea, Pisum sativum, Spinacia oleracea
J. Biol. Chem.
258
3592-3598
1983
-
-
-
-
-
-
3
3
-
-
1
3
-
7
-
-
1
-
-
5
11
1
9
-
5
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
1
3
-
-
-
1
-
5
11
1
9
-
5
-
-
-
1
-
-
-
-
-
-
-
-
-
285669
Shimakata
The procaryotic nature of the ...
Carthamus tinctorius
Arch. Biochem. Biophys.
217
144-154
1982
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
1
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2
-
1
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-
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1
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1
1
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2
-
1
-
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-
-
-
-
-
-
-
285676
Hendren
Fatty acid synthetases from Eu ...
Euglena gracilis, Euglena gracilis Z
J. Biol. Chem.
255
1504-1508
1980
-
-
-
-
-
-
-
-
-
-
1
2
-
21
-
-
-
-
-
-
1
-
4
-
1
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-
-
1
-
-
-
-
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-
-
-
-
-
-
-
-
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-
1
2
-
-
-
-
-
-
1
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
486863
Vagelos
-
Acyl group transfer (acyl carr ...
Escherichia coli
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
8
155-199
1973
-
-
-
-
-
-
-
-
-
1
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1
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1
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8
1
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1
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1
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1
-
8
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
486864
Prescott
Acyl carrier protein ...
Escherichia coli
Adv. Enzymol. Relat. Areas Mol. Biol.
36
269-311
1972
-
-
-
-
-
-
2
-
-
-
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-
1
-
-
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-
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-
5
1
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-
2
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5
1
-
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-
-
-
-
-
-
-
486870
Alberts
-
Acetyl-CoA acyl carrier protei ...
Escherichia coli, Escherichia coli B / ATCC 11303
Methods Enzymol.
14
50-53
1969
-
-
-
-
-
-
2
-
-
1
-
-
-
177
-
-
1
-
-
-
1
2
6
-
1
-
-
-
1
-
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-
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-
-
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-
-
2
-
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1
-
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1
-
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1
2
6
-
1
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-
1
-
-
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-
-
-
-
-
-
486865
Williamson
Studies on the mechanism of fa ...
Escherichia coli, Escherichia coli E26
J. Biol. Chem.
241
2326-2332
1966
-
-
-
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-
1
2
-
-
-
-
-
-
2
-
-
1
-
-
-
1
2
10
-
1
-
4
-
1
1
-
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-
-
-
-
-
-
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-
1
-
2
-
-
-
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-
-
-
-
-
1
-
-
1
2
10
-
1
-
4
-
1
1
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-
-
-
-
-
-