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Literature summary for 2.3.1.37 extracted from

  • Brown, B.L.; Kardon, J.R.; Sauer, R.T.; Baker, T.A.
    Structure of the mitochondrial aminolevulinic acid synthase, a key heme biosynthetic enzyme (2018), Structure, 26, 580-589.e4 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.37

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with pyridoxal 5'-phosphate, using 0.1 M tri-sodium citrate (pH 5.5), 20% (w/v) PEG 3000 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
DELTACT the variant lacking residues 535-548 has reduced activity (about 65% of wild type) Saccharomyces cerevisiae
R151A the mutant shows 20% of wild type activity Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinyl-CoA + glycine Saccharomyces cerevisiae
-
 5-aminolevulinate + CoA + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P09950
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA agarose resin column chromatography and Superdex S200 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + glycine
-
 Saccharomyces cerevisiae 5-aminolevulinate + CoA + CO2
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
5-aminolevulinic acid synthase
-
 Saccharomyces cerevisiae
ALAS
-
 Saccharomyces cerevisiae
Hem1
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate one enzyme subunit contains covalently bound pyridoxal 5'-phosphate whereas the second is pyridoxal 5'-phosphate-free Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the first step in heme biosynthesis Saccharomyces cerevisiae