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Literature summary for 2.3.1.37 extracted from

  • Ikushiro, H.; Nagami, A.; Takai, T.; Sawai, T.; Shimeno, Y.; Hori, H.; Miyahara, I.; Kamiya, N.; Yano, T.
    Heme-dependent inactivation of 5-aminolevulinate synthase from Caulobacter crescentus (2018), Sci. Rep., 8, 14228 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.37

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Caulobacter vibrioides

Protein Variants

Protein Variants Comment Organism
C398A the mutant shows reduced activity compared to the wild type enzyme Caulobacter vibrioides
H340A the mutant shows reduced activity compared to the wild type enzyme Caulobacter vibrioides
H340A/C398A the mutant shows reduced activity compared to the wild type enzyme Caulobacter vibrioides

Inhibitors

Inhibitors Comment Organism Structure
additional information recombinant enzyme is inactivated via a heme-mediated feedback manner, in which the essential coenzyme pyridoxal 5'-phosphate is released to form the inactive heme-bound enzyme Caulobacter vibrioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
 succinyl-CoA mutant enzyme H340A, at pH 7.5 and 25°C Caulobacter vibrioides
0.00242
-
 succinyl-CoA wild type enzyme, at pH 7.5 and 25°C Caulobacter vibrioides
0.00255
-
 succinyl-CoA mutant enzyme C398A, at pH 7.5 and 25°C Caulobacter vibrioides
0.00258
-
 succinyl-CoA mutant enzyme H340A/C398A, at pH 7.5 and 25°C Caulobacter vibrioides
3.31
-
 glycine wild type enzyme, at pH 7.5 and 25°C Caulobacter vibrioides
3.4
-
 glycine mutant enzyme C398A, at pH 7.5 and 25°C Caulobacter vibrioides
3.52
-
 glycine mutant enzyme H340A, at pH 7.5 and 25°C Caulobacter vibrioides
3.57
-
 glycine mutant enzyme H340A/C398A, at pH 7.5 and 25°C Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinyl-CoA + glycine Caulobacter vibrioides
-
 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine Caulobacter vibrioides CB15
-
 5-aminolevulinate + CoA + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides Q9A8J8
-
-
Caulobacter vibrioides CB15 Q9A8J8
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Mono Q column chromatography Caulobacter vibrioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + glycine
-
 Caulobacter vibrioides 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine
-
 Caulobacter vibrioides CB15 5-aminolevulinate + CoA + CO2
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 44273, calculated from amino acid sequence Caulobacter vibrioides

Synonyms

Synonyms Comment Organism
ALAS
-
 Caulobacter vibrioides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53
-
 melting temperature Caulobacter vibrioides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.61
-
 glycine mutant enzyme H340A, at pH 7.5 and 25°C Caulobacter vibrioides
1.61
-
 succinyl-CoA mutant enzyme H340A, at pH 7.5 and 25°C Caulobacter vibrioides
1.69
-
 glycine mutant enzyme C398A, at pH 7.5 and 25°C Caulobacter vibrioides
1.69
-
 succinyl-CoA mutant enzyme C398A, at pH 7.5 and 25°C Caulobacter vibrioides
1.73
-
 glycine wild type enzyme, at pH 7.5 and 25°C Caulobacter vibrioides
1.73
-
 succinyl-CoA wild type enzyme, at pH 7.5 and 25°C Caulobacter vibrioides
1.79
-
 glycine mutant enzyme H340A/C398A, at pH 7.5 and 25°C Caulobacter vibrioides
1.79
-
 succinyl-CoA mutant enzyme H340A/C398A, at pH 7.5 and 25°C Caulobacter vibrioides

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Caulobacter vibrioides

Expression

Organism Comment Expression
Caulobacter vibrioides the enzyme activity is negatively regulated by heme in various ways, such as the repression of enzyme gene expression, degradation of enzyme mRNA, and inhibition of mitochondrial translocation of the mammalian precursor protein down