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Literature summary for 2.3.1.37 extracted from

  • Stojanovski, B.M.; Breydo, L.; Uversky, V.N.; Ferreira, G.C.
    Murine erythroid 5-aminolevulinate synthase Truncation of a disordered N-terminal extension is not detrimental for catalysis (2016), Biochim. Biophys. Acta, 1864, 441-452 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information the truncation of the N-terminal region in mutant DELTAmALAS2 does not affect the Km values for either substrate while the kcat of the mutant enzyme, relative to that of the wild type enzyme, is increased by 22% Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
succinyl-CoA wild type enzyme, at pH 7.5 and 37°C Mus musculus
1.3
-
succinyl-CoA mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C Mus musculus
8
-
glycine wild type enzyme, at pH 7.5 and 37°C Mus musculus
11
-
glycine mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinyl-CoA + glycine Mus musculus
-
5-aminolevulinate + CoA + CO2
-
r

Organism

Organism UniProt Comment Textmining
Mus musculus P08680
-
-

Source Tissue

Source Tissue Comment Organism Textmining
erythroid cell
-
Mus musculus
-
peripheral blood cell
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + glycine
-
Mus musculus 5-aminolevulinate + CoA + CO2
-
r

Subunits

Subunits Comment Organism
homodimer 2 * 52000, 36 N-terminal amino acid-truncated enzyme, SDS-PAGE Mus musculus
homodimer 2 * 56000, native enzyme, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
ALAS2 isoform Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
49.7
-
melting temperature Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
glycine wild type enzyme, at pH 7.5 and 37°C Mus musculus
0.32
-
glycine mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C Mus musculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0291
-
glycine mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C Mus musculus
0.0312
-
glycine wild type enzyme, at pH 7.5 and 37°C Mus musculus
246
-
succinyl-CoA mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C Mus musculus
250
-
succinyl-CoA wild type enzyme, at pH 7.5 and 37°C Mus musculus