Protein Variants | Comment | Organism |
---|---|---|
additional information | the truncation of the N-terminal region in mutant DELTAmALAS2 does not affect the Km values for either substrate while the kcat of the mutant enzyme, relative to that of the wild type enzyme, is increased by 22% | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
succinyl-CoA | wild type enzyme, at pH 7.5 and 37°C | Mus musculus | |
1.3 | - |
succinyl-CoA | mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C | Mus musculus | |
8 | - |
glycine | wild type enzyme, at pH 7.5 and 37°C | Mus musculus | |
11 | - |
glycine | mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | Mus musculus | - |
5-aminolevulinate + CoA + CO2 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P08680 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythroid cell | - |
Mus musculus | - |
peripheral blood cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | - |
Mus musculus | 5-aminolevulinate + CoA + CO2 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 52000, 36 N-terminal amino acid-truncated enzyme, SDS-PAGE | Mus musculus |
homodimer | 2 * 56000, native enzyme, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
ALAS2 | isoform | Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
49.7 | - |
melting temperature | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
glycine | wild type enzyme, at pH 7.5 and 37°C | Mus musculus | |
0.32 | - |
glycine | mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0291 | - |
glycine | mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C | Mus musculus | |
0.0312 | - |
glycine | wild type enzyme, at pH 7.5 and 37°C | Mus musculus | |
246 | - |
succinyl-CoA | mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C | Mus musculus | |
250 | - |
succinyl-CoA | wild type enzyme, at pH 7.5 and 37°C | Mus musculus |