Protein Variants | Comment | Organism |
---|---|---|
A425G/Y428H/R433H/G434N/E435K/L437K | hexa variant, kcat increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type | Escherichia coli |
A425T | single variant, kcat slightly increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type | Escherichia coli |
L437Q | single variant, kcat comparable to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type | Escherichia coli |
N427H | single variant, kcat slightly increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type | Escherichia coli |
R433K/G434K/E435Q/L437Q | quadruple variant, kcat increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type | Escherichia coli |
V423I/A425P/Y428C/P432R/R433K/E435N | hexa variant, kcat increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type | Escherichia coli |
V423L/Y428R/P432E/R433I/G434N/E435Q/L437K | hepta variant, kcat increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type | Escherichia coli |
Y428I/R433Q /G434N/E435T/L437N | penta variant, kcat increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type | Escherichia coli |
Y428N/P432N/R433I/G434E/E435K/L437K | hexa variant, kcat increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
glycine | mutant Y428I/R433Q /G434N/E435T/L437N, pH 7.5, 20°C | Escherichia coli | |
0.0015 | - |
glycine | mutant N427H, pH 7.5, 20°C | Escherichia coli | |
0.0017 | - |
glycine | mutant L437Q, pH 7.5, 20°C | Escherichia coli | |
0.002 | - |
glycine | mutant Y428N/P432N/R433I/G434E/E435K/L437K, pH 7.5, 20°C | Escherichia coli | |
0.0023 | - |
glycine | mutant A425T, pH 7.5, 20°C | Escherichia coli | |
0.0023 | - |
glycine | mutant V423I/A425P/Y428C/P432R/R433K/E435N, pH 7.5, 20°C | Escherichia coli | |
0.0026 | - |
glycine | mutant A425G/Y428H/R433H/G434N/E435K/L437K, pH 7.5, 20°C | Escherichia coli | |
0.0029 | - |
glycine | mutant R433K/G434K/E435Q/L437Q, pH 7.5, 20°C | Escherichia coli | |
0.003 | - |
glycine | mutant V423L/Y428R/P432E/R433I/G434N/E435Q/L437K, pH 7.5, 20°C | Escherichia coli | |
0.011 | - |
glycine | wild-type, pH 7.5, 20°C | Escherichia coli | |
12 | - |
glycine | mutant V423L/Y428R/P432E/R433I/G434N/E435Q/L437K, pH 7.5, 20°C | Escherichia coli | |
13 | - |
glycine | mutant A425G/Y428H/R433H/G434N/E435K/L437K, pH 7.5, 20°C | Escherichia coli | |
13 | - |
glycine | mutant Y428N/P432N/R433I/G434E/E435K/L437K, pH 7.5, 20°C | Escherichia coli | |
14 | - |
glycine | mutant V423I/A425P/Y428C/P432R/R433K/E435N, pH 7.5, 20°C | Escherichia coli | |
14 | - |
glycine | wild-type, pH 7.5, 20°C | Escherichia coli | |
15 | - |
glycine | mutant N427H, pH 7.5, 20°C | Escherichia coli | |
16 | - |
glycine | mutant R433K/G434K/E435Q/L437Q, pH 7.5, 20°C | Escherichia coli | |
16 | - |
glycine | mutant Y428I/R433Q /G434N/E435T/L437N, pH 7.5, 20°C | Escherichia coli | |
24 | - |
glycine | mutant L437Q, pH 7.5, 20°C | Escherichia coli | |
25 | - |
glycine | mutant A425T, pH 7.5, 20°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | - |
Escherichia coli | 5-aminolevulinate + CoA + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALAS | - |
Escherichia coli |
sigma-aminolevulinate synthase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
glycine | wild-type, co-substrate: succinyl-CoA, pH 7.5, 20°C | Escherichia coli | |
0.03 | - |
glycine | co-substrate: succinyl-CoA, mutant L437Q, pH 7.5, 20°C | Escherichia coli | |
0.07 | - |
glycine | co-substrate: succinyl-CoA, mutant A425T, pH 7.5, 20°C | Escherichia coli | |
0.07 | - |
glycine | co-substrate: succinyl-CoA, mutant N427H, pH 7.5, 20°C | Escherichia coli | |
0.16 | - |
glycine | co-substrate: succinyl-CoA, mutant V423I/A425P/Y428C/P432R/R433K/E435N, pH 7.5, 20°C | Escherichia coli | |
0.17 | - |
glycine | co-substrate: succinyl-CoA, mutant Y428I/R433Q /G434N/E435T/L437N, pH 7.5, 20°C | Escherichia coli | |
0.2 | - |
glycine | co-substrate: succinyl-CoA, mutant A425G/Y428H/R433H/G434N/E435K/L437K, pH 7.5, 20°C | Escherichia coli | |
0.2 | - |
glycine | co-substrate: succinyl-CoA, mutant R433K/G434K/E435Q/L437Q, pH 7.5, 20°C | Escherichia coli | |
0.23 | - |
glycine | co-substrate: succinyl-CoA, mutant Y428N/P432N/R433I/G434E/E435K/L437K, pH 7.5, 20°C | Escherichia coli | |
0.31 | - |
glycine | co-substrate: succinyl-CoA, mutant V423L/Y428R/P432E/R433I/G434N/E435Q/L437K, pH 7.5, 20°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |