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Literature summary for 2.3.1.37 extracted from

  • Lendrihas, T.; Hunter, G.A.; Ferreira, G.C.
    Serine 254 enhances an induced fit mechanism in murine 5-aminolevulinate synthase (2010), J. Biol. Chem., 285, 3351-3359.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.37

Protein Variants

Protein Variants Comment Organism
S254A increase in Km value for succinyl-Coa and kcat value. Removal of the side chain hydroxyl group alters the microenvironment of the PLP cofactor and hinders succinyl-CoA binding Mus musculus
S254T decrease in kcat value without altering Km value Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 succinyl-CoA mutant S254T, 30°C, pH 7.5 Mus musculus
0.0013
-
 succinyl-CoA wild-type, 30°C, pH 7.5 Mus musculus
0.032
-
 succinyl-CoA mutant S254A, 30°C, pH 7.5 Mus musculus
18
-
 glycine mutant S254A, 30°C, pH 7.5 Mus musculus
25
-
 glycine wild-type, 30°C, pH 7.5 Mus musculus
27
-
 glycine mutant S254T, 30°C, pH 7.5 Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + glycine
-
 Mus musculus 5-aminolevulinate + CoA + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
 glycine mutant S254T, 30°C, pH 7.5 Mus musculus
0.14
-
 glycine wild-type, 30°C, pH 7.5 Mus musculus
0.27
-
 glycine mutant S254A, 30°C, pH 7.5 Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0019
-
 glycine mutant S254T, 30°C, pH 7.5 Mus musculus
0.0056
-
 glycine wild-type, 30°C, pH 7.5 Mus musculus
0.0084
-
 succinyl-CoA mutant S254A, 30°C, pH 7.5 Mus musculus
0.015
-
 glycine mutant S254A, 30°C, pH 7.5 Mus musculus
0.042
-
 succinyl-CoA mutant S254T, 30°C, pH 7.5 Mus musculus
0.11
-
 succinyl-CoA wild-type, 30°C, pH 7.5 Mus musculus