Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aminomalonate | - |
Cereibacter sphaeroides | |
Cu2+ | - |
Cereibacter sphaeroides | |
Fe2+ | - |
Cereibacter sphaeroides | |
hemin | reversible, noncompetitive | Cereibacter sphaeroides | |
p-chloromercuribenzoate | - |
Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
2 * 49000, SDS-PAGE | Cereibacter sphaeroides |
81000 | - |
gel filtration | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cereibacter sphaeroides |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 | mechanism | Cereibacter sphaeroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.22 | - |
purified enzyme | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + glycine | low activity | Cereibacter sphaeroides | ? | - |
? | |
butyryl-CoA + glycine | low activity | Cereibacter sphaeroides | ? | - |
? | |
propionyl-CoA + glycine | low activity | Cereibacter sphaeroides | ? | - |
? | |
succinyl-CoA + glycine | absolute requirement for glycine | Cereibacter sphaeroides | 5-aminolevulinate + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | enzyme utilizes acetyl-CoA, propionyl-CoA and butyryl-CoA at much lower rates | Cereibacter sphaeroides | 5-aminolevulinate + CoA + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 49000, SDS-PAGE | Cereibacter sphaeroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | fully active with 0.05 mM pyridoxal 5'-phosphate | Cereibacter sphaeroides | |
pyridoxal 5'-phosphate | weekly bound | Cereibacter sphaeroides |