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Literature summary for 2.3.1.37 extracted from

  • Jordan, P.M.; Laghai-Newton, A.
    Purification of 5-aminolevulinate synthase (1986), Methods Enzymol., 123, 435-443.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
aminomalonate
-
Cereibacter sphaeroides
Cu2+
-
Cereibacter sphaeroides
Fe2+
-
Cereibacter sphaeroides
hemin reversible, noncompetitive Cereibacter sphaeroides
p-chloromercuribenzoate
-
Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
2 * 49000, SDS-PAGE Cereibacter sphaeroides
81000
-
gel filtration Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cereibacter sphaeroides

Reaction

Reaction Comment Organism Reaction ID
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 mechanism Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.22
-
purified enzyme Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + glycine low activity Cereibacter sphaeroides ?
-
?
butyryl-CoA + glycine low activity Cereibacter sphaeroides ?
-
?
propionyl-CoA + glycine low activity Cereibacter sphaeroides ?
-
?
succinyl-CoA + glycine absolute requirement for glycine Cereibacter sphaeroides 5-aminolevulinate + CoA + CO2
-
?
succinyl-CoA + glycine enzyme utilizes acetyl-CoA, propionyl-CoA and butyryl-CoA at much lower rates Cereibacter sphaeroides 5-aminolevulinate + CoA + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 49000, SDS-PAGE Cereibacter sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate fully active with 0.05 mM pyridoxal 5'-phosphate Cereibacter sphaeroides
pyridoxal 5'-phosphate weekly bound Cereibacter sphaeroides