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Literature summary for 2.3.1.37 extracted from

  • Borthwick, I.A.; Srivastava, G.; Pirola, B.A.; May, B.K.; Elliott, W.H.
    Purification of hepatic mitochondrial 5-aminolevulinate synthase (1986), Methods Enzymol., 123, 395-401.
    View publication on PubMed
Search for more literature for 2.3.1.37

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane loosely bound Gallus gallus 5743
-
mitochondrial inner membrane loosely bound Rattus norvegicus 5743
-
mitochondrial matrix
-
 Gallus gallus 5759
-
mitochondrial matrix
-
 Rattus norvegicus 5759
-
mitochondrion
-
 Gallus gallus 5739
-
mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
succinyl-CoA + glycine Gallus gallus rate-limiting enzyme of heme biosynthesis 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine Rattus norvegicus rate-limiting enzyme of heme biosynthesis 5-aminolevulinate + CoA + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus
difficulties due to the tendency of the enzyme to form large MW aggregates and to its susceptibility to proteolytic degradation Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Gallus gallus
-
embryo
-
 Rattus norvegicus
-
liver fetal Gallus gallus
-
liver fetal Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.16
-
 purified enzyme Rattus norvegicus
0.58
-
 purified enzyme Gallus gallus

Storage Stability

Storage Stability Organism
-195°C, following an initial loss of 20% of activity due to freeze-thawing the enzyme loses no further activity for at least 6 months Gallus gallus
-195°C, following an initial loss of 20% of activity due to freeze-thawing the enzyme loses no further activity for at least 6 months Rattus norvegicus
4°C, 50 mM Tris-HCl, 100 mM glycine, 0.1 mM pyridoxal 5'-phosphate, 1 mM dithioerythritol, pH 7.6, 5 mM 5'-AMP, stable for at least 4 months Gallus gallus
4°C, 50 mM Tris-HCl, 100 mM glycine, 0.1 mM pyridoxal 5'-phosphate, 1 mM dithioerythritol, pH 7.6, 5 mM 5'-AMP, stable for at least 4 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-CoA + glycine
-
 Gallus gallus 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine
-
 Rattus norvegicus 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine rate-limiting enzyme of heme biosynthesis Gallus gallus 5-aminolevulinate + CoA + CO2
-
 ?
succinyl-CoA + glycine rate-limiting enzyme of heme biosynthesis Rattus norvegicus 5-aminolevulinate + CoA + CO2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus
37
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Gallus gallus
pyridoxal 5'-phosphate
-
 Rattus norvegicus