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Literature summary for 2.3.1.37 extracted from
- Souble delta-aminolevulinic acid synthetase of rat liver. I. Some properties of the partially purified enzyme (1972), J. Biol. Chem., 247, 4126-4131.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | required for maximal activity | Rattus norvegicus |  |
General Stability
| General Stability | Organism |
|---|---|
| partially purified enzyme requires high cation concentration, equivalent to 0.3 M NaCl for maximum stabilization | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
pyridoxal 5'-phosphate | - |
Rattus norvegicus | |
| 0.025 | - |
succinyl-CoA | - |
Rattus norvegicus | |
| 0.07 | - |
succinyl-CoA | - |
Rattus norvegicus | |
| 10 | - |
succinyl-CoA | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | partially purified enzyme requires high cation concentrations, equivalent to 0.3 M NaCl, for maximum activation, monovalent cations, i.e. Na+, K+, Li+, Rb+ or NH4+, in concentrations from 0.15 M to 0.30 M or divalent cations, i.e. Mg2+, Ca2+ or Mn2+, at lower concentrations of 0.03 M to 0.10 M are effective | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 295000 | - |
gel filtration, presence of NaCl | Rattus norvegicus |
| 500000 | - |
gel filtration, absence of NaCl | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinyl-CoA + glycine | Rattus norvegicus | rate-limiting enzyme of heme biosynthesis | 5-aminolevulinate + CoA + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.018 | - |
purified enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| succinyl-CoA + glycine | absolute requirement for glycine | Rattus norvegicus | 5-aminolevulinate + CoA + CO2 | - |
? | |
| succinyl-CoA + glycine | absolute requirement for succinyl-CoA | Rattus norvegicus | 5-aminolevulinate + CoA + CO2 | - |
? | |
| succinyl-CoA + glycine | rate-limiting enzyme of heme biosynthesis | Rattus norvegicus | 5-aminolevulinate + CoA + CO2 | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 7.6 | - |
Rattus norvegicus |
| 7.5 | - |
- |
Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8 | unstable below pH 6.8 and above pH 8.0, irreversible loss of activity | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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