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Literature summary for 2.3.1.35 extracted from

  • Elkins, J.M.; Kershaw, N.J.; Schofield, C.J.
    X-ray crystal structure of ornithine acetyltransferase from the clavulanic acid biosynthesis gene cluster (2005), Biochem. J., 385, 565-573.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant native and selenomethionine-labeled enzymes, hanging drop vapour diffusion method, 12 mg/ml protein in 1.1-1.3 M ammonium sulfate, 0.04 M ammonium phosphate, 0.1 M Tris-HCl, pH 8.0, and 5-8% v/v glycerol, and in case of SeMet-enzyme 5 mM DTT, cryoprotection by 25% glycerol and 2.0 M ammonium sulfate, X-ray diffraction structure determination and analysis at 2.8 A resolution Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N2-acetyl-L-ornithine + L-glutamate Streptomyces clavuligerus step in clavulanic acid biosynthesis via formation of arginine L-ornithine + N-acetyl-L-glutamate
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r

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus P0DJQ5 gene orf6
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Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification enzyme belongs to the N-terminal nucleophile hydrolase class of self-processing acetyltransferases Streptomyces clavuligerus

Reaction

Reaction Comment Organism Reaction ID
N2-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate substrate binding structure, oxyanion stabilization mechanism of the enzyme-substrate complex, the ping pong reaction mechanism involves Gly112 and Thr181, active site structure Streptomyces clavuligerus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme also shows low hydrolase activity Streptomyces clavuligerus ?
-
?
N2-acetyl-L-ornithine + L-glutamate
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Streptomyces clavuligerus L-ornithine + N-acetyl-L-glutamate
-
r
N2-acetyl-L-ornithine + L-glutamate step in clavulanic acid biosynthesis via formation of arginine Streptomyces clavuligerus L-ornithine + N-acetyl-L-glutamate
-
r

Subunits

Subunits Comment Organism
More 2 domain structure, the larger domain consists of an alphabetabetaalpha sandwich typical for N-terminal nucleophile Ntn enzymes Streptomyces clavuligerus
tetramer alpha2beta2 structure, the interface partly defines the active site Streptomyces clavuligerus

Synonyms

Synonyms Comment Organism
OAT
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Streptomyces clavuligerus
ornithine acetyltransferase
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Streptomyces clavuligerus