Crystallization (Comment) | Organism |
---|---|
purified recombinant native and selenomethionine-labeled enzymes, hanging drop vapour diffusion method, 12 mg/ml protein in 1.1-1.3 M ammonium sulfate, 0.04 M ammonium phosphate, 0.1 M Tris-HCl, pH 8.0, and 5-8% v/v glycerol, and in case of SeMet-enzyme 5 mM DTT, cryoprotection by 25% glycerol and 2.0 M ammonium sulfate, X-ray diffraction structure determination and analysis at 2.8 A resolution | Streptomyces clavuligerus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N2-acetyl-L-ornithine + L-glutamate | Streptomyces clavuligerus | step in clavulanic acid biosynthesis via formation of arginine | L-ornithine + N-acetyl-L-glutamate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | P0DJQ5 | gene orf6 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | enzyme belongs to the N-terminal nucleophile hydrolase class of self-processing acetyltransferases | Streptomyces clavuligerus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N2-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate | substrate binding structure, oxyanion stabilization mechanism of the enzyme-substrate complex, the ping pong reaction mechanism involves Gly112 and Thr181, active site structure | Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme also shows low hydrolase activity | Streptomyces clavuligerus | ? | - |
? | |
N2-acetyl-L-ornithine + L-glutamate | - |
Streptomyces clavuligerus | L-ornithine + N-acetyl-L-glutamate | - |
r | |
N2-acetyl-L-ornithine + L-glutamate | step in clavulanic acid biosynthesis via formation of arginine | Streptomyces clavuligerus | L-ornithine + N-acetyl-L-glutamate | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | 2 domain structure, the larger domain consists of an alphabetabetaalpha sandwich typical for N-terminal nucleophile Ntn enzymes | Streptomyces clavuligerus |
tetramer | alpha2beta2 structure, the interface partly defines the active site | Streptomyces clavuligerus |
Synonyms | Comment | Organism |
---|---|---|
OAT | - |
Streptomyces clavuligerus |
ornithine acetyltransferase | - |
Streptomyces clavuligerus |