Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus stearothermophilus |
expression in Escherichia coli | Thermotoga neapolitana |
expression in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-ornithine | - |
Geobacillus stearothermophilus | |
L-ornithine | - |
Methanocaldococcus jannaschii | |
L-ornithine | - |
Thermotoga neapolitana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
alpha-N-acetyl-L-ornithine | - |
Geobacillus stearothermophilus | |
8.1 | - |
alpha-N-acetyl-L-ornithine | - |
Thermotoga neapolitana | |
9.6 | - |
alpha-N-acetyl-L-ornithine | - |
Methanocaldococcus jannaschii | |
11.3 | - |
L-glutamate | - |
Methanocaldococcus jannaschii | |
19.2 | - |
L-glutamate | - |
Geobacillus stearothermophilus | |
27.9 | - |
L-glutamate | - |
Thermotoga neapolitana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N2-acetyl-L-ornithine + L-glutamate | Geobacillus stearothermophilus | first step in arginine biosynthesis | L-ornithine + N-acetyl-L-glutamate | - |
? | |
N2-acetyl-L-ornithine + L-glutamate | Thermotoga neapolitana | first step in arginine biosynthesis | L-ornithine + N-acetyl-L-glutamate | - |
? | |
N2-acetyl-L-ornithine + L-glutamate | Methanocaldococcus jannaschii | first step in arginine biosynthesis | L-ornithine + N-acetyl-L-glutamate | - |
? | |
N2-acetyl-L-ornithine + L-glutamate | Geobacillus stearothermophilus | bifunctional enzyme catalyzes also the fifth step of arginine biosynthesis | L-ornithine + N-acetyl-L-glutamate | - |
? | |
N2-acetyl-L-ornithine + L-glutamate | Thermotoga neapolitana | bifunctional enzyme catalyzes also the fifth step of arginine biosynthesis | L-ornithine + N-acetyl-L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Methanocaldococcus jannaschii | - |
- |
- |
Thermotoga neapolitana | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27 | - |
- |
Geobacillus stearothermophilus |
175 | - |
- |
Methanocaldococcus jannaschii |
290 | - |
- |
Thermotoga neapolitana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-glutamate | - |
Geobacillus stearothermophilus | CoA + N-acetyl-L-glutamate | - |
? | |
acetyl-CoA + L-glutamate | - |
Thermotoga neapolitana | CoA + N-acetyl-L-glutamate | - |
? | |
N2-acetyl-L-ornithine + L-glutamate | - |
Geobacillus stearothermophilus | L-ornithine + N-acetyl-L-glutamate | - |
? | |
N2-acetyl-L-ornithine + L-glutamate | - |
Thermotoga neapolitana | L-ornithine + N-acetyl-L-glutamate | - |
? | |
N2-acetyl-L-ornithine + L-glutamate | - |
Methanocaldococcus jannaschii | L-ornithine + N-acetyl-L-glutamate | - |
? | |
N2-acetyl-L-ornithine + L-glutamate | first step in arginine biosynthesis | Geobacillus stearothermophilus | L-ornithine + N-acetyl-L-glutamate | - |
? | |
N2-acetyl-L-ornithine + L-glutamate | first step in arginine biosynthesis | Thermotoga neapolitana | L-ornithine + N-acetyl-L-glutamate | - |
? | |
N2-acetyl-L-ornithine + L-glutamate | first step in arginine biosynthesis | Methanocaldococcus jannaschii | L-ornithine + N-acetyl-L-glutamate | - |
? | |
N2-acetyl-L-ornithine + L-glutamate | bifunctional enzyme catalyzes also the fifth step of arginine biosynthesis | Geobacillus stearothermophilus | L-ornithine + N-acetyl-L-glutamate | - |
? | |
N2-acetyl-L-ornithine + L-glutamate | bifunctional enzyme catalyzes also the fifth step of arginine biosynthesis | Thermotoga neapolitana | L-ornithine + N-acetyl-L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | - |
Geobacillus stearothermophilus |
heterotetramer | - |
Thermotoga neapolitana |
heterotetramer | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Geobacillus stearothermophilus |
90 | - |
- |
Thermotoga neapolitana |
95 | - |
more than 95°C | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Geobacillus stearothermophilus |
8 | - |
- |
Thermotoga neapolitana |
8 | - |
- |
Methanocaldococcus jannaschii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | - |
Geobacillus stearothermophilus |
7 | 10 | - |
Thermotoga neapolitana |
7 | 10 | - |
Methanocaldococcus jannaschii |