Literature summary for 2.3.1.31 extracted from

Goudarzi, M.; Born, T.L.
Purification and characterization of Thermotoga maritima homoserine transsuccinylase indicates it is a transacetylase (2006), Extremophiles, 10, 469-478.

Search for more literature for 2.3.1.31

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Thermotoga maritima

General Stability

General Stability	Organism
temperature inactivation readily reversible	Thermotoga maritima

Inhibitors

Inhibitors	Comment	Organism	Structure
iodoacetamide	-	Thermotoga maritima

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.017	-	malonyl-CoA	25°C, pH 7.5	Thermotoga maritima
0.044	-	propionyl-CoA	25°C, pH 7.5	Thermotoga maritima
0.051	-	succinyl-CoA	25°C, pH 7.5	Thermotoga maritima
0.068	-	isobutyryl-CoA	25°C, pH 7.5	Thermotoga maritima
0.083	-	beta-hydroxybutyryl-CoA	25°C, pH 7.5	Thermotoga maritima
0.13	-	acetyl-CoA	25°C, pH 7.5	Thermotoga maritima
0.14	-	CoA	25°C, pH 7.5	Thermotoga maritima
0.18	-	glutaryl-CoA	25°C, pH 7.5	Thermotoga maritima
0.24	-	L-homoserine	25°C, pH 7.5	Thermotoga maritima
1.7	-	O-acetylhomoserine	25°C, pH 7.5	Thermotoga maritima
280	-	D-homoserine	25°C, pH 7.5	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermotoga maritima

Reaction

Reaction	Comment	Organism	Reaction ID
acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine	enzyme utilizes a ping-pong kinetic mechanism in which the acetate group of acetyl-CoA is initially transferred to an enzyme nucleophile before subsequent transfer to homoserine	Thermotoga maritima	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + D-homoserine	-	Thermotoga maritima	CoA + O-acetyl-D-homoserine	-	?
acetyl-CoA + L-homoserine	the enzyme utilizes a ping-pong kinetic mechanism in which the acetate group of acetyl-CoA is initially transferred to an enzyme nucleophile before subsequent transfer to homoserine	Thermotoga maritima	CoA + O-acetyl-L-homoserine	-	r
beta-hydroxybutyryl-CoA + L-homoserine	-	Thermotoga maritima	CoA + beta-hydroxybutyryl-L-homoserine	-	?
glutaryl-CoA + L-homoserine	-	Thermotoga maritima	CoA + O-glutaryl-L-homoserine	-	?
isobutyryl-CoA + L-homoserine	-	Thermotoga maritima	CoA + O-isobutyryl-L-homoserine	-	?
malonyl-CoA + L-homoserine	-	Thermotoga maritima	CoA + O-malonyl-L-homoserine	-	?
propionyl-CoA + L-homoserine	-	Thermotoga maritima	CoA + O-propionyl-L-homoserine	-	?
succinyl-CoA + L-homoserine	-	Thermotoga maritima	CoA + O-succinyl-L-homoserine	-	?

Synonyms

Synonyms	Comment	Organism
TmHTS	-	Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	70	-	Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.12	-	malonyl-CoA	25°C, pH 7.5	Thermotoga maritima
0.12	-	beta-hydroxybutyryl-CoA	25°C, pH 7.5	Thermotoga maritima
0.19	-	succinyl-CoA	25°C, pH 7.5	Thermotoga maritima
0.19	-	isobutyryl-CoA	25°C, pH 7.5	Thermotoga maritima
0.25	-	glutaryl-CoA	25°C, pH 7.5	Thermotoga maritima
1.9	-	CoA	25°C, pH 7.5	Thermotoga maritima
1.9	-	O-acetylhomoserine	25°C, pH 7.5	Thermotoga maritima
2.6	-	propionyl-CoA	25°C, pH 7.5	Thermotoga maritima
16	-	D-homoserine	25°C, pH 7.5	Thermotoga maritima
23	-	acetyl-CoA	25°C, pH 7.5	Thermotoga maritima
23	-	L-homoserine	25°C, pH 7.5	Thermotoga maritima

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Release 2023.1 (January 2023)