Cloned (Comment) | Organism |
---|---|
gene cysE, recombinant overexpression of Arabidopsis thaliana AtSAT1 in Zea mays leaves lines OE1 and OE3 under control of the leaf bundle sheath cell-specific rbcS1 promoter resulting in 12fold higher SAT activity, increased S-assimilation in the leaves, and higher levels of storage protein mRNA and storage proteins, particularly the 10-kDa D-zein, during endosperm development. Quantitative RT-PCR expression analysis, the measured enzyme activity is a combination of endogenous SAT and that derived from expression of AtSAT1 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of Arabidopsis thaliana AtSAT1 in maize under control of the leaf bundle sheath cell-specific rbcS1 promoter to determine the impact on seed storage protein expression. The transgenic events exhibit up to 12fold higher SAT activity without negative impact on growth. S-assimilation is increased in the leaves of SAT overexpressing plants, followed by higher levels of storage protein mRNA and storage proteins, particularly the 10-kDa D-zein, during endosperm development. This zein is known to impact the level of Met stored in kernels. The elite event with the highest expression of AtSAT1 shows 1.40fold increase in kernel Met. Efficacy of increasing maize nutritional value by SAT overexpression without apparent yield loss. Maternal overexpression of SAT in vegetative tissues is necessary for high-Met zein accumulation. Moreover, SAT overcomes the shortage of S-amino acids that limits the expression and accumulation of high-Met zeins during kernel development. Mature kernels of AtSAT1 overexpressing maize show changes in zein expression profile. The measured enzyme activity is a combination of endogenous SAT and that derived from expression of AtSAT1 | Zea mays |
additional information | the transgenic events in Zea mays leaves exhibit up to 12fold higher SAT activity without negative impact on growth. S-assimilation is increased in the leaves of SAT overexpressing plants, followed by higher levels of storage protein mRNA and storage proteins, particularly the 10-kDa D-zein, during endosperm development. This zein is known to impact the level of Met stored in kernels. The elite event with the highest expression of AtSAT1 shows 1.40fold increase in kernel Met | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | AtSAT1 protein lacks a transit peptide | Arabidopsis thaliana | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | Arabidopsis thaliana | - |
CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | Zea mays | - |
CoA + O-acetyl-L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q42588 | - |
- |
Zea mays | A0A3L6DLJ7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kernel | - |
Zea mays | - |
leaf | - |
Arabidopsis thaliana | - |
leaf | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | - |
Arabidopsis thaliana | CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | - |
Zea mays | CoA + O-acetyl-L-serine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtSAT1 | - |
Arabidopsis thaliana |
SAT | - |
Arabidopsis thaliana |
SAT | - |
Zea mays |
SAT1 | - |
Arabidopsis thaliana |
SAT1 | - |
Zea mays |
serine acetyltransferase | - |
Arabidopsis thaliana |
serine acetyltransferase | - |
Zea mays |
serine acetyltransferase 1 | - |
Arabidopsis thaliana |
serine acetyltransferase 1 | - |
Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Arabidopsis thaliana | |
acetyl-CoA | - |
Zea mays |
General Information | Comment | Organism |
---|---|---|
malfunction | overexpression of Arabidopsis thaliana serine acetyltransferase in maize leaves increases seed-specific methionine-rich zeins, overexpression has the effect of, not only enhancing S-assimilation, but also, indirectly impacting expression of high-Met seed storage proteins | Zea mays |
metabolism | serine acetyltransferase (SAT) is a key control point for S-assimilation leading to Cys and Met biosynthesis | Arabidopsis thaliana |
metabolism | serine acetyltransferase (SAT) is a key control point for S-assimilation leading to Cys and Met biosynthesis. Met synthesis in maize is not strictly controlled by the enzyme cystathionine gamma-synthase (CGS) | Zea mays |
physiological function | serine acetyltransferase (SAT) is a key control point for S-assimilation leading to Cys and Met biosynthesis | Arabidopsis thaliana |
physiological function | serine acetyltransferase (SAT) is a key control point for S-assimilation leading to Cys and Met biosynthesis. SAT overexpression is known to enhance S-assimilation without negative impact on plant growth, it increases seed-specific methionine-rich D-zeins | Zea mays |