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Literature summary for 2.3.1.30 extracted from
- Characterization of serine acetyltransferase (CysE) from methicillin-resistant Staphylococcus aureus and inhibitory effect of two natural products on CysE (2019), Microb. Pathog., 131, 218-226 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cysE, recombinant expression of C-terminally His-tagged in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain NovaBlue using vector pET29b | Staphylococcus aureus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D29A | site-directed mutagenesis, the mutant shows reduced activity compared to wild-type | Staphylococcus aureus |
| D94A | site-directed mutagenesis, the mutant shows reduced activity compared to wild-type | Staphylococcus aureus |
| H95A | site-directed mutagenesis, the mutant shows reduced activity compared to wild-type | Staphylococcus aureus |
| K128A | site-directed mutagenesis, the mutant shows reduced activity compared to wild-type | Staphylococcus aureus |
| N148A | site-directed mutagenesis, the mutant shows reduced activity compared to wild-type | Staphylococcus aureus |
| R129A | site-directed mutagenesis, soluble Asp29Ala fails to be expressed | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (1aR,1bS,4aR,7bS,8R,9R,9aS)-4a,7b,9-trihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1,1a,1b,4,4a,5,7a,7b,8,9-decahydro-9aH-cyclopropa[3,4]benzo[1,2-e]azulen-9a-yl acetate | - |
Staphylococcus aureus |  |
| 11beta,12alpha,17-trihydroxy-5beta,9beta,10alpha-12,16-epoxyabieta-8(14),13(15)-dien-16-one | - |
Staphylococcus aureus | |
| 7beta,11alpha,17-trihydroxyhelioscopinolide E | - |
Staphylococcus aureus | |
| 8,11beta,14beta-trihydroxy-5beta,8alpha,9beta,10alpha,12beta-12,16-epoxyabiet-13(15)-en-16-one | - |
Staphylococcus aureus | |
| 8,14alpha-dihydroxy-5beta,9beta,10alpha,12alpha-12,16-epoxyabiet-13(15)-ene-11,16-dione | - |
Staphylococcus aureus | |
| 8,14beta,17-trihydroxy-5beta,8alpha,9beta,10alpha-12,16-epoxyabieta-11,13(15)-dien-16-one | - |
Staphylococcus aureus | |
| additional information | analysis of cytotoxicity of compounds 8,14alpha-dihydroxy-5beta,9beta,10alpha,12alpha-12,16-epoxyabiet-13(15)-ene-11,16-dione and 8,14beta,17-trihydroxy-5beta,8alpha,9beta,10alpha-12,16-epoxyabieta-11,13(15)-dien-16-one, inhibition types of different inhibitors on MRSA CysE, overview; ligand molecular docking study | Staphylococcus aureus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.296 | - |
acetyl-CoA | pH 7.5, 37°C, recombinant wild-type enzyme | Staphylococcus aureus | |
| 0.459 | - |
L-serine | pH 7.5, 37°C, recombinant wild-type enzyme | Staphylococcus aureus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-serine | Staphylococcus aureus | - |
CoA + O-acetyl-L-serine | - |
? | |
| acetyl-CoA + L-serine | Staphylococcus aureus ATCC 33591 | - |
CoA + O-acetyl-L-serine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
MRSA strain | - |
| Staphylococcus aureus ATCC 33591 | - |
MRSA strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His-tagged from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Staphylococcus aureus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000304 | - |
substrate L-serine, pH 7.5, 37°C, recombinant mutant D94A | Staphylococcus aureus |
| 0.0000812 | - |
substrate L-serine, pH 7.5, 37°C, recombinant mutant R129A | Staphylococcus aureus |
| 0.000141 | - |
substrate L-serine, pH 7.5, 37°C, recombinant mutant N148A | Staphylococcus aureus |
| 0.000218 | - |
substrate L-serine, pH 7.5, 37°C, recombinant mutant H95A | Staphylococcus aureus |
| 0.000696 | - |
substrate L-serine, pH 7.5, 37°C, recombinant mutant K128A | Staphylococcus aureus |
| 0.00389 | - |
substrate L-serine, pH 7.5, 37°C, recombinant wild-type enzyme | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-serine | - |
Staphylococcus aureus | CoA + O-acetyl-L-serine | - |
? | |
| acetyl-CoA + L-serine | - |
Staphylococcus aureus ATCC 33591 | CoA + O-acetyl-L-serine | - |
? | |
| additional information | serine acetyltransferase activity of CysE protein is detected by both DTNB [5,5'-dithiobis-(2-nitrobenzoic acid)] colorimetric assay and HPLC assay. Post-docking interactions between active site residues of protein (MRSA CysE) with ligand (L-Ser) | Staphylococcus aureus | ? | - |
- |
|
| additional information | serine acetyltransferase activity of CysE protein is detected by both DTNB [5,5'-dithiobis-(2-nitrobenzoic acid)] colorimetric assay and HPLC assay. Post-docking interactions between active site residues of protein (MRSA CysE) with ligand (L-Ser) | Staphylococcus aureus ATCC 33591 | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SAT | - |
Staphylococcus aureus |
| serine acetyltransferase | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
recombinant enzyme | Staphylococcus aureus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | over 40% of maximal activity within this range | Staphylococcus aureus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.36 | - |
L-serine | pH 7.5, 37°C, recombinant wild-type enzyme | Staphylococcus aureus | |
| 1.683 | - |
acetyl-CoA | pH 7.5, 37°C, recombinant wild-type enzyme | Staphylococcus aureus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
recombinant enzyme | Staphylococcus aureus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | over 40% of maximal activity within this range | Staphylococcus aureus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Staphylococcus aureus | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.02983 | - |
pH 7.5, 37°C, recombinant wild-type enzyme | Staphylococcus aureus | 7beta,11alpha,17-trihydroxyhelioscopinolide E | |
| 0.07184 | - |
pH 7.5, 37°C, recombinant wild-type enzyme | Staphylococcus aureus | 8,14alpha-dihydroxy-5beta,9beta,10alpha,12alpha-12,16-epoxyabiet-13(15)-ene-11,16-dione | |
| 0.07184 | - |
pH 7.5, 37°C, recombinant wild-type enzyme | Staphylococcus aureus | 8,14beta,17-trihydroxy-5beta,8alpha,9beta,10alpha-12,16-epoxyabieta-11,13(15)-dien-16-one | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | homology model building and molecular docking of CysE, overview. Six residues (Asp29, Asp94, His95, Lys128, Arg129 and Asn148) of CysE are showed to be bound with L-Ser | Staphylococcus aureus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.96 | - |
L-serine | pH 7.5, 37°C, recombinant wild-type enzyme | Staphylococcus aureus | |
| 5.68 | - |
acetyl-CoA | pH 7.5, 37°C, recombinant wild-type enzyme | Staphylococcus aureus | |
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