Cloned (Comment) | Organism |
---|---|
recombinant expression | Brucella abortus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in apo state and in complex with coenzyme A, hanging drop vapour diffusion methd, mixing of 14 mg/ml protein in 50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 5% glycerol, and 5 mM 2-mercaptoethanol with reservoir solution containing 7% w/v PEG 1000, 7% w/v PEG 3350, 5% v/v MPD, 0.12 methylene glycol, 0.1 M Tris-HCl, pH 7.4, and 50 mM MgCl2, 16°C, X-ray diffraction structure determination and analysis at 1.96 A and 1.87 A resolution, respectively | Brucella abortus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-cysteine | competitive inhibition versus L-serine, noncompetitive inhibition versus acetyl-CoA | Brucella abortus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, overview | Brucella abortus | |
0.1275 | - |
L-serine | recombinant enzyme, pH 8.0, temperature not specified in the publication | Brucella abortus | |
0.1923 | - |
L-serine | recombinant enzyme, pH 8.0, temperature not specified in the publication, in presence of 5 mM L-cysteine | Brucella abortus | |
0.2783 | - |
L-serine | recombinant enzyme, pH 8.0, temperature not specified in the publication, in presence of 10 mM L-cysteine | Brucella abortus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
trimeric enzyme, gel filtration | Brucella abortus |
181000 | - |
hexameric enzyme, gel filtration and dynamic light scattering | Brucella abortus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | Brucella abortus | - |
CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | Brucella abortus 2308 A | - |
CoA + O-acetyl-L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brucella abortus | C4IRW0 | gene CysE | - |
Brucella abortus 2308 A | C4IRW0 | gene CysE | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme by nickel affinity chromatography and gel filtration | Brucella abortus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + L-serine = CoA + O-acetyl-L-serine | there is no conformational difference in the enzyme between the apo and the substrate-bound states, indicating lock and key binding and the absence of an induced fit mechanism | Brucella abortus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-serine | - |
Brucella abortus | CoA + O-acetyl-L-serine | - |
? | |
acetyl-CoA + L-serine | - |
Brucella abortus 2308 A | CoA + O-acetyl-L-serine | - |
? | |
additional information | coenzyme A binds to the C-terminal region, making mostly hydrophobic contacts from the center of the active site extending up to the surface of the protein. There is no conformational difference in the enzyme between the apo and the substrate-bound states, indicating lock and key binding and the absence of an induced fit mechanism | Brucella abortus | ? | - |
? | |
additional information | coenzyme A binds to the C-terminal region, making mostly hydrophobic contacts from the center of the active site extending up to the surface of the protein. There is no conformational difference in the enzyme between the apo and the substrate-bound states, indicating lock and key binding and the absence of an induced fit mechanism | Brucella abortus 2308 A | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | in dynamic light scattering and in crystal structure | Brucella abortus |
trimer | trimer in gel filtration | Brucella abortus |
Synonyms | Comment | Organism |
---|---|---|
SAT | - |
Brucella abortus |
serine acetyltransferase | - |
Brucella abortus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Brucella abortus |