Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
S726F | the mutant shows 3% of wild type activity | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0701 | - |
(S)-methylmalonyl-CoA | wild type enzyme, at pH 7.0 and 30°C | Mycobacterium tuberculosis | |
0.1176 | - |
malonyl-CoA | mutant enzyme S726F, at pH 7.0 and 30°C | Mycobacterium tuberculosis | |
0.2386 | - |
(S)-methylmalonyl-CoA | mutant enzyme S726F, at pH 7.0 and 30°C | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + 17-(4-hydroxyphenyl)heptadecanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH | Mycobacterium tuberculosis | - |
C35-(phenol)carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O | - |
? | |
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + 17-(4-hydroxyphenyl)heptadecanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH | Mycobacterium tuberculosis H37Rv | - |
C35-(phenol)carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O | - |
? | |
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + 19-(4-hydroxyphenyl)-nonadecanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH | Mycobacterium tuberculosis | - |
C37-(phenol)carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O | - |
? | |
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + 19-(4-hydroxyphenyl)-nonadecanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH | Mycobacterium tuberculosis H37Rv | - |
C37-(phenol)carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O | - |
? | |
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + docosanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH | Mycobacterium tuberculosis | - |
C34-carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O | - |
? | |
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + docosanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH | Mycobacterium tuberculosis H37Rv | - |
C34-carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O | - |
? | |
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + icosanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH | Mycobacterium tuberculosis | - |
C32-carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O | - |
? | |
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + icosanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH | Mycobacterium tuberculosis H37Rv | - |
C32-carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Resource Q column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + 17-(4-hydroxyphenyl)heptadecanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH | - |
Mycobacterium tuberculosis | C35-(phenol)carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O | - |
? | |
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + 17-(4-hydroxyphenyl)heptadecanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH | - |
Mycobacterium tuberculosis H37Rv | C35-(phenol)carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O | - |
? | |
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + 19-(4-hydroxyphenyl)-nonadecanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH | - |
Mycobacterium tuberculosis | C37-(phenol)carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O | - |
? | |
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + 19-(4-hydroxyphenyl)-nonadecanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH | - |
Mycobacterium tuberculosis H37Rv | C37-(phenol)carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O | - |
? | |
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + docosanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH | - |
Mycobacterium tuberculosis | C34-carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O | - |
? | |
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + docosanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH | - |
Mycobacterium tuberculosis H37Rv | C34-carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O | - |
? | |
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + icosanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH | - |
Mycobacterium tuberculosis | C32-carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O | - |
? | |
3 malonyl-CoA + 2 (S)-methylmalonyl-CoA + icosanoyl-[(phenol)carboxyphthiodiolenone synthase] + 5 NADPH | - |
Mycobacterium tuberculosis H37Rv | C32-carboxyphthiodiolenone-[(phenol)carboxyphthiodiolenone synthase] + 5 CoA + 5 NADP+ + 5 CO2 + 2 H2O | - |
? | |
additional information | the enzyme shows a strict specificity for malonyl-CoA | Mycobacterium tuberculosis | ? | - |
? | |
additional information | the enzyme shows a strict specificity for malonyl-CoA | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 163000, PpsE, SDS-PAGE | Mycobacterium tuberculosis |
? | x * 170000, PpsB, SDS-PAGE | Mycobacterium tuberculosis |
? | x * 200000, PpsA, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
PpsA | - |
Mycobacterium tuberculosis |
PpsB | - |
Mycobacterium tuberculosis |
PpsE | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
(S)-methylmalonyl-CoA | mutant enzyme S726F, at pH 7.0 and 30°C | Mycobacterium tuberculosis | |
0.102 | - |
malonyl-CoA | mutant enzyme S726F, at pH 7.0 and 30°C | Mycobacterium tuberculosis | |
0.22 | - |
(S)-methylmalonyl-CoA | wild type enzyme, at pH 7.0 and 30°C | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | the first step of phthiocerol synthesis is catalyzed by PpsA protein. The lauroyl diketide from PpsA protein is transferred to the beta-ketoacyl ACP synthase domain of PpsB protein (formation of the diol moiety of phthiocerol). PpsE catalyzes the final step in the synthesis of phthiocerol | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0838 | - |
(S)-methylmalonyl-CoA | mutant enzyme S726F, at pH 7.0 and 30°C | Mycobacterium tuberculosis | |
0.9659 | - |
malonyl-CoA | mutant enzyme S726F, at pH 7.0 and 30°C | Mycobacterium tuberculosis | |
3.1339 | - |
(S)-methylmalonyl-CoA | wild type enzyme, at pH 7.0 and 30°C | Mycobacterium tuberculosis |