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Literature summary for 2.3.1.29 extracted from
- Kinetics of the exchange reaction catalyzed by 2-amino-3-ketobutyrate CoA ligase (2015), Front. Biol., 10, 503-507.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expresssion in Escherichia coli | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of the exchange of pro-R hydrogen of glycine with protons in the medium by the enzyme | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43117.15, recombinant enzyme, mass spectrometry, x * 43000, recombinant enzyme, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + glycine | Escherichia coli | condensation reaction | CoA + L-2-amino-3-oxobutanoate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by gel filtration | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + glycine | condensation reaction | Escherichia coli | CoA + L-2-amino-3-oxobutanoate | - |
? | |
| additional information | the enzyme catalyzes the exchange of pro-R hydrogen of glycine with protons in the medium | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43117.15, recombinant enzyme, mass spectrometry, x * 43000, recombinant enzyme, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2-amino-3-ketobutyrate CoA ligase | - |
Escherichia coli |
| KBL | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Escherichia coli | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | 2-amino-3-ketobutyrate CoA ligase of Escherichia coli is a member of the alpha-oxoamine synthase family | Escherichia coli |
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Release 2023.1 (January 2023)
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