Cloned (Comment) | Organism |
---|---|
expressed in Sf9 insect cells | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
coumermycin A1 | completely inhibited by 0.2 mM | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hyperacetylated histone-DNA complex + NAD+ + H2O | Drosophila melanogaster | - |
deacetylated nuclease-resistant histone-DNA complex + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q9VK34 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity column chromatography and Superdex S200 gel filtration | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hyperacetylated histone-DNA complex + NAD+ + H2O | - |
Drosophila melanogaster | deacetylated nuclease-resistant histone-DNA complex + ? | - |
? | |
[histone H2B]-N6-acetyl-L-lysine20 + NAD+ + H2O | - |
Drosophila melanogaster | [histone H2B]-L-lysine20 + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? | |
[histone H2B]-N6-acetyl-L-lysine5 + NAD+ + H2O | - |
Drosophila melanogaster | [histone H2B]-L-lysine5 + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? | |
[histone H3]-N6-acetyl-L-lysine14 + NAD+ + H2O | - |
Drosophila melanogaster | [histone H3]-L-lysine14 + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? | |
[histone H3]-N6-acetyl-L-lysine18 + NAD+ + H2O | - |
Drosophila melanogaster | [histone H3]-L-lysine18 + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? | |
[histone H3]-N6-acetyl-L-lysine23 + NAD+ + H2O | - |
Drosophila melanogaster | [histone H3]-L-lysine23 + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? | |
[histone H3]-N6-acetyl-L-lysine9 + NAD+ + H2O | - |
Drosophila melanogaster | [histone H3]-L-lysine9 + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? | |
[histone H4]-N6-acetyl-L-lysine12 + NAD+ + H2O | - |
Drosophila melanogaster | [histone H4]-L-lysine12 + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? | |
[histone H4]-N6-acetyl-L-lysine16 + NAD+ + H2O | - |
Drosophila melanogaster | [histone H4]-L-lysine16 + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? | |
[histone H4]-N6-acetyl-L-lysine5 + NAD+ + H2O | - |
Drosophila melanogaster | [histone H4]-L-lysine5 + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? | |
[histone H4]-N6-acetyl-L-lysine8 + NAD+ + H2O | weak activity | Drosophila melanogaster | [histone H4]-L-lysine8 + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 116000, SDS-PAGE | Drosophila melanogaster |
Synonyms | Comment | Organism |
---|---|---|
NAD-dependent histone deacetylase | - |
Drosophila melanogaster |
Sir2 | - |
Drosophila melanogaster |
SIRT1 | - |
Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme does not repress transcription with either naked DNA templates or chromatin assembled from native (and mostly unacetylated) histones | Drosophila melanogaster |