Protein Variants | Comment | Organism |
---|---|---|
H135A | the kcat/Km profile for the mutant exhibits no pH dependence over the range tested (pH 5.4-9.4), with kcat/Km values 2 orders of magnitude lower than wild type at high pH values | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[protein]-N6-acetyl-L-lysine + NAD+ | Saccharomyces cerevisiae | - |
[protein]-N6-[1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl]-L-lysine + nicotinamide | - |
? | |
[protein]-N6-acetyl-L-lysine + NAD+ + H2O | Saccharomyces cerevisiae | overall reaction | [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? | |
[protein]-N6-[1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl]-L-lysine + H2O | Saccharomyces cerevisiae | - |
[protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
KSTGGAcKAPRKQ + NAD+ + H2O | - |
Saccharomyces cerevisiae | KSTGGKAPRKQ + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? | |
[histone H3]-N6-acetyl-L-lysine + NAD+ + H2O | - |
Saccharomyces cerevisiae | [histone H3]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? | |
[protein]-N6-acetyl-L-lysine + NAD+ | - |
Saccharomyces cerevisiae | [protein]-N6-[1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl]-L-lysine + nicotinamide | - |
? | |
[protein]-N6-acetyl-L-lysine + NAD+ + H2O | overall reaction | Saccharomyces cerevisiae | [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose + nicotinamide | - |
? | |
[protein]-N6-[1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl]-L-lysine + H2O | - |
Saccharomyces cerevisiae | [protein]-L-lysine + 2''-O-acetyl-ADP-D-ribose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HST2 | - |
Saccharomyces cerevisiae |
Sir2 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
KSTGGAcKAPRKQ | mutant enzyme H135A, at pH 7.5 and 25°C | Saccharomyces cerevisiae | |
2.2 | - |
KSTGGAcKAPRKQ | wild type enzyme, at pH 7.5 and 25°C | Saccharomyces cerevisiae |