Literature summary for 2.3.1.28 extracted from

Van Dorst, B.; Mehta, J.; Rouah-Martin, E.; Backeljau, J.; De Coen, W.; Eeckhout, D.; De Jaeger, G.; Blust, R.; Robbens, J.
Selection of scFv phages specific for chloramphenicol acetyl transferase (CAT), as alternatives for antibodies in CAT detection assays (2012), J. Appl. Toxicol., 32, 783-789.

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Application

Application	Comment	Organism
molecular biology	single-chain variable fragment (scFv) phages are selected with affinity for CAT. Surface plasmon resonance analyses shows that the tested scFv phages have an affinity for CAT with a dissociation constant (Kd) around 1 microM. The selected scFv phages can be used as capture elements in a highly sensitive sandwich ELISA to detect CAT concentration as low as 0.1 ng/ml or 4 pM	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Synonyms

Synonyms	Comment	Organism
CAT	-	Escherichia coli

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Release 2023.1 (January 2023)