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Literature summary for 2.3.1.28 extracted from

  • Chen, M.W.; Nagarajan, V.
    Chloramphenicol acetyltransferase, a cytoplasmic protein is incompatible for export from Bacillus subtilis (1993), J. Bacteriol., 175, 5697-5700.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information Bacillus subtilis cells expressing a hybrid protein (LvsSS-Cat) consisting of the Bacillus amyloliquefaciens levansucrose signal peptide fused to Bacillus pumilus chloramphenicol acetyltransferase are unable to export cat protein into the growth medium. A series of tripartite protein fusion is constructed by inserting various length of the cat sequences between the levansucrase signal peptide and staphylococcal protein A or Escherichia coli alkaline phosphatase. Biochemical characterization of the various Cat protein fusion reveales that multiple regions in the cat protein are causing the export defect Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Bacillus subtilis 5737
-

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + chloramphenicol
-
Bacillus subtilis CoA + chloramphenicol 3-acetate
-
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