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Literature summary for 2.3.1.28 extracted from

  • Shaw, W.V.
    Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria (1975), Methods Enzymol., 43, 737-755.
    View publication on PubMed
Search for more literature for 2.3.1.28

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid)
-
 Agrobacterium tumefaciens
5,5'-dithiobis(2-nitrobenzoic acid)
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + chloramphenicol Enterococcus faecalis inducible enzyme CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol Streptococcus pneumoniae inducible enzyme CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol Staphylococcus sp. inducible enzyme CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol Agrobacterium tumefaciens inducible enzyme CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol Escherichia coli catabolite repression of CAT synthesis is mediated by a mechanism involving cyclic adenosine 5'-monophosphate CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol Escherichia coli all known R factors carrying the CAT gene in enteric bacteria mediate constitutive synthesis of the enzyme CoA + chloramphenicol 3-acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Enterococcus faecalis
-
-
-
Escherichia coli
-
-
-
Staphylococcus sp.
-
-
-
Streptococcus pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli
-
 Staphylococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli
additional information
-
-
 Staphylococcus sp.

Storage Stability

Storage Stability Organism
-20 C, activity in elution buffer is stable for at least 1 year Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + chloramphenicol
-
 Escherichia coli CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol
-
 Enterococcus faecalis CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol
-
 Streptococcus pneumoniae CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol
-
 Staphylococcus sp. CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol
-
 Agrobacterium tumefaciens CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol inducible enzyme Enterococcus faecalis CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol inducible enzyme Streptococcus pneumoniae CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol inducible enzyme Staphylococcus sp. CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol inducible enzyme Agrobacterium tumefaciens CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol catabolite repression of CAT synthesis is mediated by a mechanism involving cyclic adenosine 5'-monophosphate Escherichia coli CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol all known R factors carrying the CAT gene in enteric bacteria mediate constitutive synthesis of the enzyme Escherichia coli CoA + chloramphenicol 3-acetate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
-
 Escherichia coli
75
-
 stable Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Escherichia coli