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Literature summary for 2.3.1.276 extracted from

  • Zhang, Z.; Shimizu, Y.; Kawarabayasi, Y.
    Characterization of the amino acid residues mediating the unique amino-sugar-1-phosphate acetyltransferase activity of the archaeal ST0452 protein (2015), Extremophiles, 19, 417-427.
    View publication on PubMed
Search for more literature for 2.3.1.276

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21-Codon Plus(DE3)-RIL Sulfurisphaera tokodaii

Protein Variants

Protein Variants Comment Organism
H308A specific activity is 7.7% compared to the wild-type enzyme Sulfurisphaera tokodaii
K340A specific activity is 63.3% compared to the wild-type enzyme Sulfurisphaera tokodaii
K377A specific activity is 82.6% compared to the wild-type enzyme Sulfurisphaera tokodaii
additional information C-terminal deletion mutants DC005 and DC011 (deletion of the C-terminal 5 or 11 residues of the ST0452 protein) shows 20% and 38% less galactosamine-1-phosphate acetyltransferase activity than the wild-type ST0452 protein. The mutant enzyme DC011 (deletion of the C-terminal 11 residues of the ST0452 protein) shows little thermal stability at 80°C. The C-terminal domain of the ST0452 protein, with its LbetaH structure, appears to be essential for the formation of its trimeric form and, in turn, the high stability of the entire ST0452 protein. The deletion mutant enzymes DC021, DC031, DC041, DC071 and DC121, are produced in an insoluble form or aggregated immediately after purification. Mutant enzymes DC051 and DC171 can be expressed in a soluble form. Mutant enzyme DC051 becomes completely insoluble after 5 min treatment at 60°C, while mutant enzyme DC171 is insoluble after 5 min treatment at 70 °C Sulfurisphaera tokodaii
N331A specific activity is 3.1% compared to the wild-type enzyme Sulfurisphaera tokodaii
Y311A specific activity is 3.3% compared to the wild-type enzyme Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.52
-
 acetyl-CoA pH 7.5, 80°C, mutant enzyme DC005 with a deletion of the C-terminal 5 residues of the ST0452 protein Sulfurisphaera tokodaii
0.63
-
 acetyl-CoA pH 7.5, 80°C, wild-type enzyme Sulfurisphaera tokodaii
0.66
-
 alpha-D-galactosamine 1-phosphate pH 7.5, 80°C, mutant enzyme DC011 with a deletion of the C-terminal 11 residues of the ST0452 protein Sulfurisphaera tokodaii
0.84
-
 alpha-D-galactosamine 1-phosphate pH 7.5, 80°C, mutant enzyme DC005 with a deletion of the C-terminal 5 residues of the ST0452 protein Sulfurisphaera tokodaii
1.55
-
 acetyl-CoA pH 7.5, 80°C, mutant enzyme DC011 with a deletion of the C-terminal 11 residues of the ST0452 protein Sulfurisphaera tokodaii
1.71
-
 alpha-D-galactosamine 1-phosphate pH 7.5, 80°C, wild-type enzyme Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + alpha-D-galactosamine 1-phosphate Sulfurisphaera tokodaii
-
 CoA + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q975F9
-
-
Sulfurisphaera tokodaii 7 Q975F9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.23
-
 pH 7.5, 80°C, mutant enzyme N331A Sulfurisphaera tokodaii
1.31
-
 pH 7.5, 80°C, mutant enzyme Y311A Sulfurisphaera tokodaii
3
-
 pH 7.5, 80°C, mutant enzyme H308A Sulfurisphaera tokodaii
25.3
-
 pH 7.5, 80°C, mutant enzyme K340A Sulfurisphaera tokodaii
33
-
 pH 7.5, 80°C, mutant enzyme K337A Sulfurisphaera tokodaii
40
-
 pH 7.5, 80°C, wild-type enzyme enzyme Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + alpha-D-galactosamine 1-phosphate
-
 Sulfurisphaera tokodaii CoA + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
trimer the C-terminal domain of the ST0452 protein, with its LbetaH structure, appears to be essential for the formation of its trimeric form Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
Gal-1-P AcTase
-
 Sulfurisphaera tokodaii
galactosamine-1-phosphate acetyltransferase
-
 Sulfurisphaera tokodaii
ST0452
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 assay at Sulfurisphaera tokodaii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 mutant enzyme DC005 shows the same thermostability as wild-type ST0452 protein, whereas mutant enzyme DC011 denatures and becomes insoluble form by 5-min treatment at 80 °C. The C-terminal domain of the ST0452 protein, with its LbetaH structure, appears to be essential for the formation of its trimeric form and, in turn, the high stability of the entire ST0452 protein Sulfurisphaera tokodaii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.6
-
 alpha-D-galactosamine 1-phosphate pH 7.5, 80°C, mutant enzyme DC011 with a deletion of the C-terminal 11 residues of the ST0452 protein Sulfurisphaera tokodaii
17.9
-
 alpha-D-galactosamine 1-phosphate pH 7.5, 80°C, mutant enzyme DC005 with a deletion of the C-terminal 5 residues of the ST0452 protein Sulfurisphaera tokodaii
69.7
-
 alpha-D-galactosamine 1-phosphate pH 7.5, 80°C, wild-type enzyme Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sulfurisphaera tokodaii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
19.6
-
 alpha-D-galactosamine 1-phosphate pH 7.5, 80°C, mutant enzyme DC011 with a deletion of the C-terminal 11 residues of the ST0452 protein Sulfurisphaera tokodaii
21.5
-
 alpha-D-galactosamine 1-phosphate pH 7.5, 80°C, mutant enzyme DC005 with a deletion of the C-terminal 5 residues of the ST0452 protein Sulfurisphaera tokodaii
40.8
-
 alpha-D-galactosamine 1-phosphate pH 7.5, 80°C, wild-type enzyme Sulfurisphaera tokodaii