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Literature summary for 2.3.1.275 extracted from

  • Hara, Y.; Seki, M.; Matsuoka, S.; Hara, H.; Yamashita, A.; Matsumoto, K.
    Involvement of PlsX and the acyl-phosphate dependent sn-glycerol-3-phosphate acyltransferase PlsY in the initial stage of glycerolipid synthesis in Bacillus subtilis (2008), Genes Genet. Syst., 83, 433-442.
    View publication on PubMed
Search for more literature for 2.3.1.275

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus subtilis two-hybrid examinations show that PlsY is associated with PlsX protein which produces acyl-phosphate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information two-hybrid examinations show that PlsY is associated with PlsX protein which produces acyl-phosphate Bacillus subtilis ?
-
 ?
palmitoleoyl phosphate + glycerol-3-phosphate
-
 Bacillus subtilis 1-palmitoleoyl-2-lyso-sn-glycerol 3-phosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
multimer it is suggested that PlsY exists as a multimer to achieve an effecient lysophosphatidic acid in Bacillus subtilis Bacillus subtilis

Synonyms

Synonyms Comment Organism
glycerol-3-phosphate acyltransferase
-
 Bacillus subtilis
GPAT
-
 Bacillus subtilis
PlsY uses PslX produced acyl-phosphate as acyl-residue donor Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 assay at Bacillus subtilis

General Information

General Information Comment Organism
malfunction in Bacillus subtilis growth arrests after repression of either plsY or plsX are overcome by expression of Escherichia coli plsB, encoding an acyl-acylcarrier protein-dependent GPAT Bacillus subtilis
malfunction membrane fractions from Bacillus subtilis cells catalyze palmitoylphosphate-dependent acylation of [14C]-labeled glycerol-3-phosphate to synthesize [14C]-labeled lysophosphatidic acid, whereas those cells with deleted plsY do not Bacillus subtilis
malfunction simultaneous expression of plsY and plsX (gene product which produces acyl-phosphate as an acyl-donor) suppresses the glycerol requirement of a strict glycerol auxotrophic derivative of the Escherichia coli plsB26 mutant, although either one alone does not Bacillus subtilis