BRENDA - Enzyme Database
show all sequences of 2.3.1.269

Structural insights into the mechanism of the membrane integral N-acyltransferase step in bacterial lipoprotein synthesis

Wiktor, M.; Weichert, D.; Howe, N.; Huang, C.Y.; Olieric, V.; Boland, C.; Bailey, J.; Vogeley, L.; Stansfeld, P.J.; Buddelmeijer, N.; Wang, M.; Caffrey, M.; Nat. Commun. 8, 15952 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli C41(DE3) cells
Escherichia coli
expressed in Escherichia coli C41(DE3) cells
Pseudomonas aeruginosa
Crystallization (Commentary)
Crystallization
Organism
using 30% (w/v) PEG-500 DME, 0.1 M sodium citrate pH 5.0 and 0.1 M sodium acetate
Pseudomonas aeruginosa
using 8% (w/v) 2-methyl-2,4-pentanediol, 0.1 M MES pH 6.0 and 0.4 M ammonium citrate
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
C387A
inactive
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Escherichia coli
16020
-
membrane
-
Pseudomonas aeruginosa
16020
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P23930
-
-
Pseudomonas aeruginosa
Q9ZI86
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography and Superdex 200 gel filtration
Escherichia coli
Ni-NTA column chromatography and Superdex 200 gel filtration
Pseudomonas aeruginosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
phosphatidylethanolamine + [apolipoprotein FSL-1]-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
748746
Escherichia coli
1-lyso-phosphatidylethanolamine + [lipoprotein FSL-1]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
phosphatidylethanolamine + [apolipoprotein FSL-1]-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
748746
Pseudomonas aeruginosa
1-lyso-phosphatidylethanolamine + [lipoprotein FSL-1]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli C41(DE3) cells
Escherichia coli
expressed in Escherichia coli C41(DE3) cells
Pseudomonas aeruginosa
Crystallization (Commentary) (protein specific)
Crystallization
Organism
using 30% (w/v) PEG-500 DME, 0.1 M sodium citrate pH 5.0 and 0.1 M sodium acetate
Pseudomonas aeruginosa
using 8% (w/v) 2-methyl-2,4-pentanediol, 0.1 M MES pH 6.0 and 0.4 M ammonium citrate
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C387A
inactive
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Escherichia coli
16020
-
membrane
-
Pseudomonas aeruginosa
16020
-
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography and Superdex 200 gel filtration
Escherichia coli
Ni-NTA column chromatography and Superdex 200 gel filtration
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
phosphatidylethanolamine + [apolipoprotein FSL-1]-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
748746
Escherichia coli
1-lyso-phosphatidylethanolamine + [lipoprotein FSL-1]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
phosphatidylethanolamine + [apolipoprotein FSL-1]-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
748746
Pseudomonas aeruginosa
1-lyso-phosphatidylethanolamine + [lipoprotein FSL-1]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
Other publictions for EC 2.3.1.269
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747441
da Silva
The role of apolipoprotein N- ...
Neisseria meningitidis, Neisseria meningitidis MC58
Br. J. Pharmacol.
174
2247-2260
2017
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748746
Wiktor
Structural insights into the ...
Escherichia coli, Pseudomonas aeruginosa
Nat. Commun.
8
15952
2017
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2
2
1
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2
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4
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2
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749188
Noland
Structural insights into lipo ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
114
E6044-E6053
2017
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1
2
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1
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1
1
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748683
Gelis-Jeanvoine
Residues located on membrane- ...
Escherichia coli
Mol. Microbiol.
95
692-705
2015
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-
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-
8
-
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1
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1
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1
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1
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8
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1
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747413
Bruelle
Lipoproteins of slow-growing ...
Mycobacterium tuberculosis variant bovis
BMC Microbiol.
13
223
2013
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1
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5
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748084
Narita
Overexpression of LolCDE allo ...
Escherichia coli
J. Bacteriol.
193
4832-4840
2011
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1
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1
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2
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6
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6
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748169
Hillmann
Kinetics and phospholipid spe ...
Escherichia coli
J. Biol. Chem.
286
27936-27946
2011
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1
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1
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3
1
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3
1
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747066
Buddelmeijer
The essential Escherichia col ...
Escherichia coli
Biochemistry
49
341-346
2010
-
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2
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1
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1
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2
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1
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748164
Tschumi
Identification of apolipoprot ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis Rv2051c
J. Biol. Chem.
284
27146-27156
2009
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1
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7
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748078
Vidal-Ingigliardi
Identification of essential r ...
Escherichia coli
J. Bacteriol.
189
4456-4464
2007
-
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8
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1
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4
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748159
Robichon
Depletion of apolipoprotein N ...
Escherichia coli
J. Biol. Chem.
280
974-983
2005
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2
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748140
Gupta
Characterization of a tempera ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
268
16551-16556
1993
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1
1
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747801
Gupta
Identification and subcellula ...
Escherichia coli, Escherichia coli JE 5505
FEMS Microbiol. Lett.
78
37-41
1991
4
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7
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3
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7
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