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Literature summary for 2.3.1.268 extracted from
- Increase ethyl acetate production in Saccharomyces cerevisiae by genetic engineering of ethyl acetate metabolic pathway (2019), J. Ind. Microbiol. Biotechnol., 46, 801-808 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene eat1, quantitative RT-PCR enzyme expression analysis in engineered strain CLy12a derivatives | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | in Saccharomyces cerevisiae, the genes encoding phosphopantothenate-cysteine ligase, acetyl-CoA synthetase, and alcohol acetyltransferase are overexpressed by inserting the combined strong promoter PGK1p and the terminator PGK1t. The ethyl acetate levels of all engineered strains are enhanced. The final engineered strain CLy12a-ATF1-ACS2-CAB2 has an ethyl acetate yield of max. 610.26 mg/l, and the yield of higher alcohols is significantly decreased. The modification of ethyl acetate metabolic pathway is extremely important for the high level production of ethyl acetate in Saccharomyces cerevisiae | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethanol + acetyl-CoA | Saccharomyces cerevisiae | - |
ethyl acetate + CoA | - |
? |  |
| ethanol + acetyl-CoA | Saccharomyces cerevisiae ATCC 204508 | - |
ethyl acetate + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P53208 | - |
- |
| Saccharomyces cerevisiae ATCC 204508 | P53208 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethanol + acetyl-CoA | - |
Saccharomyces cerevisiae | ethyl acetate + CoA | - |
? | |
| ethanol + acetyl-CoA | - |
Saccharomyces cerevisiae ATCC 204508 | ethyl acetate + CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alcohol acetyltransferase | - |
Saccharomyces cerevisiae |
| eat1 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
in vivo assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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