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Literature summary for 2.3.1.263 extracted from

  • Fonknechten, N.; Perret, A.; Perchat, N.; Tricot, S.; Lechaplais, C.; Vallenet, D.; Vergne, C.; Zaparucha, A.; Le Paslier, D.; Weissenbach, J.; Salanoubat, M.
    A conserved gene cluster rules anaerobic oxidative degradation of L-ornithine (2009), J. Bacteriol., 191, 3162-3167.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.263

Cloned(Commentary)

Cloned (Comment) Organism
expression as a His-tagged protein Acetoanaerobium sticklandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 CoA pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate Acetoanaerobium sticklandii
0.035
-
 2-Amino-4-oxopentanoate pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate Acetoanaerobium sticklandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13930
-
 2 * 13930 + 2 * 51450, calculated from sequence Acetoanaerobium sticklandii
14000
-
 2 * 50000 + 2 * 14000, SDS-PAGE Acetoanaerobium sticklandii
50000
-
 2 * 50000 + 2 * 14000, SDS-PAGE Acetoanaerobium sticklandii
51450
-
 2 * 13930 + 2 * 51450, calculated from sequence Acetoanaerobium sticklandii
136000
-
 gel filtration Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + 2-amino-4-oxopentanoate Acetoanaerobium sticklandii catalyzes a step in the ornithine fermentation pathway acetyl-CoA + D-alanine
-
 r

Organism

Organism UniProt Comment Textmining
Acetoanaerobium sticklandii C1FW07 and C1FW06 C1FW07: beta subunit, C1FW06: alpha subunit. No significant homology with any known enzyme family
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acetoanaerobium sticklandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + 2-amino-4-oxopentanoate catalyzes a step in the ornithine fermentation pathway Acetoanaerobium sticklandii acetyl-CoA + D-alanine
-
 r
CoA + 2-amino-4-oxopentanoate strict substrate specificity. No activity with other proteogenic amino acid nor with propional-CoA, crotonyl-CoA, succinyl-CoA, butyryl-CoA or malonyl-CoA. In the reverse reaction formation of CoA-SH is observed only in the presence of D-alanine as substrate and not in that of the L-isomer Acetoanaerobium sticklandii acetyl-CoA + D-alanine
-
 r

Subunits

Subunits Comment Organism
heterotetramer 2 * 50000 + 2 * 14000, SDS-PAGE Acetoanaerobium sticklandii
tetramer 2 * 13930 + 2 * 51450, calculated from sequence Acetoanaerobium sticklandii

Synonyms

Synonyms Comment Organism
AKP thiolase
-
 Acetoanaerobium sticklandii
AKPT
-
 Acetoanaerobium sticklandii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4
-
 2-Amino-4-oxopentanoate pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate Acetoanaerobium sticklandii
3.5
-
 CoA pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate Acetoanaerobium sticklandii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate strictly pyridoxal 5'-phosphate-dependent enzyme Acetoanaerobium sticklandii

General Information

General Information Comment Organism
metabolism catalyzes a step in the ornithine fermentation pathway Acetoanaerobium sticklandii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
97
-
 2-Amino-4-oxopentanoate pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate Acetoanaerobium sticklandii
190
-
 CoA pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate Acetoanaerobium sticklandii