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Literature summary for 2.3.1.26 extracted from

  • Yu, C.; Kennedy, N.J.; Chang, C.C.Y.; Rothblatt, J.A.
    Molecular cloning and characterization of two isoforms of Saccharomyces cerevisiae acyl-CoA:sterol acyltransferase (1996), J. Biol. Chem., 271, 24157-24163.
    View publication on PubMed
Search for more literature for 2.3.1.26

Cloned(Commentary)

Cloned (Comment) Organism
expression in sat1 sat2 mutant cells of Saccharomyces cerevisiae Homo sapiens
two genes, SAT1 and SAT2 encode isozymes of the enzyme Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
CI-976 cell-free extract of sat1 and sat2 cells: more than 95% inhibition at 0.2 mM, intact sat1 and sat2 cells: approximately 75-80% inhibition at 0.2 mM Homo sapiens
CI-976 cell-free extract of wild-type cells: approximately 90% inhibition at 0.1 mM and more than 95% inhibition at 0.2 mM, intact wild-type cells: approximately 75-80% inhibition at 0.2 mM Saccharomyces cerevisiae
DuP 128
-
 Homo sapiens
DuP 128 approximately 50% inhibition at 0.03-0.04 mM Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 recombinant enzyme Homo sapiens
71600
-
 deduced from amino acid sequence data Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Saccharomyces cerevisiae P53629
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein there are six potential N-linked glycosylation sites in sat1, two of which are conserved in sat2 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 when yeast-expressed enzyme activity is measured in the presence of ergosterol liposomes, specific activity is reduced more than 80%, recombinant enzyme is active but unable to esterify ergosterol efficiently Homo sapiens
0.0009
-
 recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oleoyl-CoA + cholesterol
-
 Saccharomyces cerevisiae CoA + cholesteryl oleate
-
 ?
oleoyl-CoA + cholesterol recombinant enzyme Homo sapiens CoA + cholesteryl oleate
-
 ?
oleoyl-CoA + ergosterol endogenous ergosterol as substrate or exogenous ergosterol in the form of ergosterol/phosphatidylcholine vesicles as substrate Saccharomyces cerevisiae CoA + ergosteryl oleate
-
 ?
oleoyl-CoA + ergosterol recombinant enzyme: restores ergosteryl oleate formation in vivo to only approximately 8% of that catalysed by yeast enzyme in wild-type cells Homo sapiens CoA + ergosteryl oleate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 assay at Saccharomyces cerevisiae