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Literature summary for 2.3.1.259 extracted from

  • Chen, J.Y.; Liu, L.; Cao, C.L.; Li, M.J.; Tan, K.; Yang, X.; Yun, C.H.
    Structure and function of human Naa60 (NatF), a Golgi-localized bi-functional acetyltransferase (2016), Sci. Rep., 6, 31425.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.259

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with acetyl-coenzyme A or coenzyme A, hanging drop vapor diffusion method, using 10 mM Tris pH 8.0, 75 mM NaCl, 0.5% (v/v) glycerol, 3% (v/v) Tacsimate pH 4.0, and 7.5% (w/v) polyethylene glycol 3350 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D81A APPLICATION the mutant shows about 2fold increased activity compared to the wild type enzyme Homo sapiens
D83A the mutant shows about 2.8fold increased activity compared to the wild type enzyme Homo sapiens
E37A the mutant shows wild type activity Homo sapiens
E80A the mutant shows about 3.6fold increased activity compared to the wild type enzyme Homo sapiens
F34A inactive Homo sapiens
H138A inactive Homo sapiens
H139F inactive Homo sapiens
K79A the mutant shows about 20% reduced activity compared to the wild type enzyme Homo sapiens
K79Q the mutant shows about 1.8fold increased activity compared to the wild type enzyme Homo sapiens
K79R the mutant shows about 1.5fold increased activity compared to the wild type enzyme Homo sapiens
L140A the mutant shows about 20% reduced activity compared to the wild type enzyme Homo sapiens
N143A the mutant shows about 60% reduced activity compared to the wild type enzyme Homo sapiens
Y165A inactive Homo sapiens
Y38A inactive Homo sapiens
Y97A inactive Homo sapiens
Y97F inactive Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Homo sapiens 5794
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9H7X0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + MKGKEEKEGGAR
-
 Homo sapiens Nalpha-acetyl-MKGKEEKEGGAR + CoA
-
 ?
additional information the enzyme also harbors lysine Nepsilon-acetyltransferase activity to catalyze the acetylation of lysine epsilon-amine Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
Naa60
-
 Homo sapiens
NatF
-
 Homo sapiens