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Literature summary for 2.3.1.257 extracted from

  • Magin, R.; Liszczak, G.; Marmorstein, R.
    The molecular basis for histone H4- and H2A-specific amino-terminal acetylation by NatD (2015), Structure, 23, 332-341.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.257

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in binary complex with acetyl-CoA, and in ternary complex with CoA and an N-terminal histone H4/H2A, hanging drop vapor diffusion method, using 20% (w/v) PEG 3350, 200 mM sodium citrate tribasic dihydrate, 100 mM citric acid, pH 4.0 or 20% (w/) PEG 3350 and 200 mM sodium malonate, pH 6.0 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D127A/E129A the mutant shows significantly decreased catalytic efficiency (1.6-18fold) compared to the wild type enzyme Homo sapiens
E139Q inactive Homo sapiens
T174A the mutant shows wild type catalytic efficiency Homo sapiens
W90A the mutant exhibits markedly decreased catalytic efficiency (2.8fold) compared to the wild type enzyme Homo sapiens
Y136A the mutant shows significantly decreased catalytic efficiency (1.6-1.8fold) compared to the wild type enzyme Homo sapiens
Y136F the catalytic efficiency of the mutant is modestly affected compared to the wild type enzyme Homo sapiens
Y138A the mutant shows significantly decreased catalytic efficiency (1.6-1.8fold) compared to the wild type enzyme Homo sapiens
Y211A the mutant shows wild type catalytic efficiency Homo sapiens
Y85A the mutant exhibits markedly decreased catalytic efficiency (5.8fold) compared to the wild type enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + an N-terminal-L-seryl-[histone H2A] Homo sapiens
-
 an N-terminal-Nalpha-acetyl-L-seryl-[histone H2A] + CoA
-
 ?
acetyl-CoA + an N-terminal-L-seryl-[histone H4] Homo sapiens
-
 an N-terminal-Nalpha-acetyl-L-seryl-[histone H4] + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q86UY6
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel resin column chromatography and HiTrap Q column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + an N-terminal-L-seryl-[histone H2A]
-
 Homo sapiens an N-terminal-Nalpha-acetyl-L-seryl-[histone H2A] + CoA
-
 ?
acetyl-CoA + an N-terminal-L-seryl-[histone H4]
-
 Homo sapiens an N-terminal-Nalpha-acetyl-L-seryl-[histone H4] + CoA
-
 ?

Synonyms

Synonyms Comment Organism
Naa40p
-
 Homo sapiens
NAT4
-
 Homo sapiens
NatD
-
 Homo sapiens
Patt1
-
 Homo sapiens