Cloned (Comment) | Organism |
---|---|
cloning of V5-tagged hNaa15p, hNaa25p, and hNaa35p from HEK-293 cell genomic DNA, expression of His-tagged and maltose binding protein-tagged subunits hNaa15p, hNaa10p, and hNaa50p in Escherichia coli, efficient expression of MBP-hNaa15p requires coexpression of pDC952, a plasmid carrying the Escherichia coli argU gene | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
ribosome | the HYPK-hNatA interaction may occur at the ribosome, active translation is not obligatory for the interaction to occur. Thus, HYPK stably associates with hNatA, common binding to the ribosomes | Homo sapiens | 5840 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + PCNP protein | Homo sapiens | i.e. PEST proteolytic signal-containing nuclear protein | CoA + Nalpha-acetyl-PCNP protein | - |
? | |
acetyl-CoA + peptide | Homo sapiens | - |
CoA + Nalpha-acetylpeptide | - |
? | |
additional information | Homo sapiens | endogenous HYPK, a Huntingtin (Htt)-interacting protein, is a stable interactor of NatA, the C terminus of hNaa15p of NatA specifically interacts directly with HYPK, no interaction with hNaa25p of hNatB and hNaa35p of hNatC | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged and maltose binding protein-tagged subunits hNaa15p, hNaa10p, and hNaa50p from Escherichia coli by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + ACTH peptide | 17 amino acids are identical to the adrenocorticotropin (ACTH) peptide sequence, the ACTH-derived lysines are replaced by arginines to minimize any potential interference by Nalpha-acetylation | Homo sapiens | CoA + ? | - |
? | |
acetyl-CoA + PCNP protein | i.e. PEST proteolytic signal-containing nuclear protein | Homo sapiens | CoA + Nalpha-acetyl-PCNP protein | - |
? | |
acetyl-CoA + peptide | - |
Homo sapiens | CoA + Nalpha-acetylpeptide | - |
? | |
acetyl-CoA + SESSSKSRWGRPVGRRRRPVRVYP | high-mobility-group protein A1 sequence | Homo sapiens | CoA + Ac-SESSSKSRWGRPVGRRRRPVRVYP | - |
? | |
additional information | endogenous HYPK, a Huntingtin (Htt)-interacting protein, is a stable interactor of NatA, the C terminus of hNaa15p of NatA specifically interacts directly with HYPK, no interaction with hNaa25p of hNatB and hNaa35p of hNatC | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NatA | - |
Homo sapiens |
NatA protein Nalpha-terminal-acetyltransferase complex | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Homo sapiens |