Literature summary for 2.3.1.254 extracted from

Alves, S.; Neiri, L.; Rodrigues Chaves, S.; Trindade, V.D.; Manon, S.; Dominguez, V.; Pintado, B.; Jonckheere, V.; Van Damme, P.; Silva, R.D.; Aldabe, R.; Corte-Real, M.
N-terminal acetylation modulates Bax targeting to mitochondria (2018), Int. J. Biochem. Cell Biol., 95, 35-42 .

Search for more literature for 2.3.1.254

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of Naa25-/- MEF cells and of NAA25-knockout mice	Mus musculus
additional information	generation yeast naa20DELTA cells, W303 naa20DELTA, from wild-type strain W303-1B	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Saccharomyces cerevisiae	5737	-
cytoplasm	-	Mus musculus	5737	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]	Saccharomyces cerevisiae	-	an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]	Mus musculus	-	an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]	Mus musculus C57BL/6	-	an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]	Saccharomyces cerevisiae ATCC 204508	-	an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]	Saccharomyces cerevisiae	-	an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]	Mus musculus	-	an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]	Mus musculus C57BL/6	-	an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]	Saccharomyces cerevisiae ATCC 204508	-	an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]	Saccharomyces cerevisiae	-	an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]	Mus musculus	-	an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]	Mus musculus C57BL/6	-	an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]	Saccharomyces cerevisiae ATCC 204508	-	an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]	Saccharomyces cerevisiae	-	an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]	Mus musculus	-	an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]	Mus musculus C57BL/6	-	an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]	Saccharomyces cerevisiae ATCC 204508	-	an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	P61600 AND Q8BWZ3	subunits Naa20 and Naa25 of enzyme complex NatB	-
Mus musculus C57BL/6	P61600 AND Q8BWZ3	subunits Naa20 and Naa25 of enzyme complex NatB	-
Saccharomyces cerevisiae	Q06504 AND Q12387	subunits Nat3p and Mdm20p of enzyme complex NatB	-
Saccharomyces cerevisiae ATCC 204508	Q06504 AND Q12387	subunits Nat3p and Mdm20p of enzyme complex NatB	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
MEF cell	-	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]	-	Saccharomyces cerevisiae	an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]	-	Mus musculus	an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]	-	Mus musculus C57BL/6	an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]	-	Saccharomyces cerevisiae ATCC 204508	an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]	-	Saccharomyces cerevisiae	an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]	-	Mus musculus	an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]	-	Mus musculus C57BL/6	an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]	-	Saccharomyces cerevisiae ATCC 204508	an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]	-	Saccharomyces cerevisiae	an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]	-	Mus musculus	an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]	-	Mus musculus C57BL/6	an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]	-	Saccharomyces cerevisiae ATCC 204508	an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]	-	Saccharomyces cerevisiae	an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]	-	Mus musculus	an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]	-	Mus musculus C57BL/6	an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA	-	?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]	-	Saccharomyces cerevisiae ATCC 204508	an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA	-	?
acetyl-CoA + N-terminal L-methionyl-L-aspartyl-[Bax]	heterologous expression of human Bax in mouse embryonic fibroblasts (MEFs). Recombinant human Bax is N-terminally (Nt-)acetylated by Naa20 and that Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of MEF cells. Human Bax displays the N-terminal Met-Asp amino-acid sequence	Mus musculus	an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[Bax] + CoA	-	?
acetyl-CoA + N-terminal L-methionyl-L-aspartyl-[Bax]	heterologous expression of human Bax in Saccharomyces cerevisiae. Recombinant human Bax is N-terminal (Nt-)acetylated by yNaa20p and that Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of yeast. Human Bax displays the N-terminal Met-Asp amino-acid sequence	Saccharomyces cerevisiae	an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[Bax] + CoA	-	?
acetyl-CoA + N-terminal L-methionyl-L-aspartyl-[Bax]	heterologous expression of human Bax in mouse embryonic fibroblasts (MEFs). Recombinant human Bax is N-terminally (Nt-)acetylated by Naa20 and that Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of MEF cells. Human Bax displays the N-terminal Met-Asp amino-acid sequence	Mus musculus C57BL/6	an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[Bax] + CoA	-	?
acetyl-CoA + N-terminal L-methionyl-L-aspartyl-[Bax]	heterologous expression of human Bax in Saccharomyces cerevisiae. Recombinant human Bax is N-terminal (Nt-)acetylated by yNaa20p and that Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of yeast. Human Bax displays the N-terminal Met-Asp amino-acid sequence	Saccharomyces cerevisiae ATCC 204508	an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[Bax] + CoA	-	?

Subunits

Subunits	Comment	Organism
heterodimer	the NatB enzymatic complex is constituted of two subunits, the NAA20 catalytic subunit and the accessory subunit NAA25	Mus musculus
heterodimer	the NatB enzymatic complex is constituted of two subunits, the Naa3p catalytic subunit and the accessory subunit MDM20	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
MDM20	-	Saccharomyces cerevisiae
Mdm20p	-	Saccharomyces cerevisiae
N-terminal acetyltransferase B complex catalytic subunit NAA20	UniProt	Mus musculus
NAA20	-	Mus musculus
NAA25	-	Mus musculus
NAT3	-	Saccharomyces cerevisiae
Nat3p	-	Saccharomyces cerevisiae
natB	-	Saccharomyces cerevisiae
natB	-	Mus musculus

Cofactor

Cofactor	Comment	Organism	Structure
acetyl-CoA	-	Saccharomyces cerevisiae
acetyl-CoA	-	Mus musculus

General Information

General Information	Comment	Organism
malfunction	Naa25 inactivation is embryonic lethal in homozygocity. Recombinant Bax accumulates in the mitochondria of Naa25-/- MEF cells, but does not promote cytochrome c release, suggesting that an additional step is required for full activation of Bax	Mus musculus
malfunction	recombinant Bax accumulates in the mitochondria of yeast naa20DELTA (W303 naa20DELTA), but does not promote cytochrome c release, suggesting that an additional step is required for full activation of Bax	Saccharomyces cerevisiae
additional information	the NatB enzymatic complex is constituted of two subunits, the NAA20 catalytic subunit and the accessory subunit NAA25	Mus musculus
additional information	the NatB enzymatic complex is constituted of two subunits, the Naa3p catalytic subunit and the accessory subunit MDM20	Saccharomyces cerevisiae
physiological function	recombinant human Bax expressed in MEF cells is N-terminal (Nt-)acetylated by Naa20p. Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of the cells. N-terminal acetylation modulates Bax targeting to mitochondria. Contribution of Bax Nt-acetylation to its regulation	Mus musculus
physiological function	recombinant human Bax expressed in strain BY4743 is N-terminally (Nt-)acetylated by yNaa20p. Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of yeast. N-terminal acetylation modulates Bax targeting to mitochondria. Contribution of Bax Nt-acetylation to its regulation	Saccharomyces cerevisiae