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Literature summary for 2.3.1.254 extracted from

  • Evjenth, R.; Hole, K.; Karlsen, O.; Ziegler, M.; Amesen, T.; Lillehaug, J.
    Human Naa50p (Nat5/San) displays both protein Nalpha- and Nepsilon-acetyltransferase activity (2009), J. Biol. Chem., 284, 31122-31129.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.254

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
R84A the mutant has more than 80% less activity than the wild type enzyme Homo sapiens
Y124F the mutant has about 90% less activity than the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0069
-
 acetyl-CoA at pH 8.5 and 37°C Homo sapiens
0.079
-
 MLGPEGGRWGRPVGRRRRRPVRVYP at pH 8.5 and 37°C Homo sapiens
0.091
-
 MLDPEEGGRWGRPVGRRRRRPVRVYP at pH 8.5 and 37°C Homo sapiens
0.185
-
 MIGPEEGGRWGRPVGRRRRRPVRVYP at pH 8.5 and 37°C Homo sapiens
0.19
-
 MLALISRRWGRPVGRRRRRPVRVYP at pH 8.5 and 37°C Homo sapiens
0.32
-
 MLGTGPARWGRPVGRRRRRPVRVYP at pH 8.5 and 37°C Homo sapiens
0.416
-
 MLGTEEGGRWGRPVGRRRRRPVRVYP at pH 8.5 and 37°C Homo sapiens
0.46
-
 MLRPEEGGRWGRPVGRRRRRPVRVYP at pH 8.5 and 37°C Homo sapiens
0.478
-
 MLLPEEGGRWGRPVGRRRRRPVRVYP at pH 8.5 and 37°C Homo sapiens
3.734
-
 MFGPEEGGRWGRPVGRRRRRPVRVYP at pH 8.5 and 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + MFGPEEGGRWGRPVGRRRRRPVRVYP
-
 Homo sapiens CoA + N-acetyl-MFGPEEGGRWGRPVGRRRRRPVRVYP
-
 ?
acetyl-CoA + MIGPEEGGRWGRPVGRRRRRPVRVYP
-
 Homo sapiens CoA + N-acetyl-MIGPEEGGRWGRPVGRRRRRPVRVYP
-
 ?
acetyl-CoA + MLALISRRWGRPVGRRRRRPVRVYP
-
 Homo sapiens CoA + N-acetyl-MLALISRRWGRPVGRRRRRPVRVYP
-
 ?
acetyl-CoA + MLDPEEGGRWGRPVGRRRRRPVRVYP
-
 Homo sapiens CoA + N-acetyl-MLDPEEGGRWGRPVGRRRRRPVRVYP
-
 ?
acetyl-CoA + MLGPEGGRWGRPVGRRRRRPVRVYP
-
 Homo sapiens CoA + N-acetyl-MLGPEGGRWGRPVGRRRRRPVRVYP
-
 ?
acetyl-CoA + MLGTEEGGRWGRPVGRRRRRPVRVYP
-
 Homo sapiens CoA + N-acetyl-MLGTEEGGRWGRPVGRRRRRPVRVYP
-
 ?
acetyl-CoA + MLGTGPARWGRPVGRRRRRPVRVYP
-
 Homo sapiens CoA + N-acetyl-MLGTGPARWGRPVGRRRRRPVRVYP
-
 ?
acetyl-CoA + MLLPEEGGRWGRPVGRRRRRPVRVYP
-
 Homo sapiens CoA + N-acetyl- MLLPEEGGRWGRPVGRRRRRPVRVYP
-
 ?
acetyl-CoA + MLRPEEGGRWGRPVGRRRRRPVRVYP
-
 Homo sapiens CoA + N-acetyl-MLRPEEGGRWGRPVGRRRRRPVRVYP
-
 ?
additional information no activity with MKEEVKGRWGRPVGRRRRRPVRVYP and MDELFPLRWGRPVGRRRRRPVRVYP Homo sapiens ?
-
 ?
additional information the enzyme also shows lysine Nepsilon-acetyltransferase activity with histone 4 Homo sapiens ?
-
 ?
additional information the enzyme preferably acetylates oligopeptides with N-termini Met-Leu-Xxx-Pro. Furthermore, the enzyme autoacetylates lysines 34, 37, and 140 in vitro Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
Naa50p
-
 Homo sapiens
NAT5/SAN
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.038
-
 MLGTGPARWGRPVGRRRRRPVRVYP at pH 8.5 and 37°C Homo sapiens
0.093
-
 MLGTEEGGRWGRPVGRRRRRPVRVYP at pH 8.5 and 37°C Homo sapiens
0.11
-
 MLALISRRWGRPVGRRRRRPVRVYP at pH 8.5 and 37°C Homo sapiens
0.12
-
 MLGPEGGRWGRPVGRRRRRPVRVYP at pH 8.5 and 37°C Homo sapiens
0.12
-
 MLDPEEGGRWGRPVGRRRRRPVRVYP at pH 8.5 and 37°C Homo sapiens
0.18
-
 MIGPEEGGRWGRPVGRRRRRPVRVYP at pH 8.5 and 37°C Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme Naa50p is important for chromosome segregation Homo sapiens