Crystallization (Comment) | Organism |
---|---|
molecular dynamics simulation and comparison of Salmonella typhimurium and Escherichia coli enzymes. In vitro lipase activity of the Salmonella enzyme is 15-20fold higher than for the Escherichia coli enzyme. Protein stability correlates inversely with activity: the Escherichia coli PagP equilibrium free energy is 2fold higher | Salmonella enterica subsp. enterica serovar Typhimurium |
molecular dynamics simulation and comparison of Salmonella typhimurium and Escherichia coli enzymes. In vitro lipase activity of the Salmonella enzyme is 15-20fold higher than for the Escherichia coli enzyme. Protein stability correlates inversely with activity: the Escherichia coli PagP equilibrium free energy is 2fold higher | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
K91S | mutation leads to 2fold reduction in the protein stability by about 3.45 kcal/mol | Salmonella enterica subsp. enterica serovar Typhimurium |
L57Q | mutation leads to destabilizization by about 3.5 kcal/mol | Escherichia coli |
Q57L | presence of Leu confers an added 2.2 kcal/mol stabilization | Salmonella enterica subsp. enterica serovar Typhimurium |
S91K | mutation leads to stabilizization by about 3.0 kcal/mol | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P37001 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |