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Literature summary for 2.3.1.250 extracted from

  • Tanaka, K.; Okabayashi, K.; Asashima, M.; Perrimon, N.; Kadowaki, T.
    The evolutionarily conserved porcupine family is involved in the processing of the Wnt family (2000), Eur. J. Biochem., 267, 4300-4311.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene MG61, DNA and amino acid sequence determination and analysis, sequence comparisons Homo sapiens
gene mom-1, DNA and amino acid sequence determination and analysis, sequence comparisons Caenorhabditis elegans
gene Porc, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in HEK-293 cells with or without co-expression of Wg Drosophila melanogaster
gene porc, expression of the enzyme tagged with three HA epitopes at amino acid 28. Capped porc, porc3HA28, DELTA34, DELTA66, and DELTA110 RNA are prepared by in vitro transcription and RNA is injected into Drosophila embryos Drosophila melanogaster
gene Xporc, DNA and amino acid sequence determination and analysis, sequence comparisons Xenopus laevis
single gene Mporc, variants by alternative splicing, DNA and amino acid sequence determination and analysis, sequence comparisons, co-expression in COS-1 cells of HA-tagged Mporc and either Myc-tagged Wnt or partial laminin beta chain, recombinant expression in HEK-293 cells with or without co-expression of Drosophila melanogaster Wnt variant Wg Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information generation of three different deletion mutants of the enzyme lacking residues 1-34, 1-66, or 1-110 (DELTA34, DELTA66, and DELTA110), the mutants show affeced N-glycosylation of Wingless protein, because the mutant enzymes cannot bind to Wingless, overview Drosophila melanogaster
additional information rescue of Drosophila melanogaster porc embryos by Mporc RNA injection Mus musculus
additional information rescue of Drosophila porc embryos by Mus musculus Mporc RNA injection Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane a multispanning transmembrane protein Drosophila melanogaster 5789
-
endoplasmic reticulum membrane the enzyme has multiple transmembrane domains Homo sapiens 5789
-
endoplasmic reticulum membrane the enzyme has multiple transmembrane domains Mus musculus 5789
-
endoplasmic reticulum membrane the enzyme has multiple transmembrane domains Xenopus laevis 5789
-
endoplasmic reticulum membrane the enzyme has multiple transmembrane domains Caenorhabditis elegans 5789
-
endoplasmic reticulum membrane the enzyme has multiple transmembrane domains, Drosophila Porc is localized at the endoplasmic reticulum when expressed in Drosophila cells as an epitope-tagged protein Drosophila melanogaster 5789
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(9Z)-hexadec-9-enoyl-CoA + [Wnt3]-L-serine Drosophila melanogaster
-
CoA + [Wnt3]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt5]-L-serine Drosophila melanogaster
-
CoA + [Wnt5]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine Drosophila melanogaster the enzyme catalyzes the palmitoylation of Wnt Wingless or Wg CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine Homo sapiens
-
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine Mus musculus
-
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine Drosophila melanogaster
-
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine Xenopus laevis
-
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine Caenorhabditis elegans
-
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
additional information Drosophila melanogaster enzyme Porc functions on the N-terminal 24-amino acid domain (83-106) of Wg, binding of Porc with Wg is essential for its activity ?
-
?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans Q22329 gene mom-1; gene mom-1
-
Drosophila melanogaster
-
gene porcupine, i.e. porc
-
Drosophila melanogaster Q9VWV9 gene Porc or CG6205
-
Homo sapiens Q9H237 gene porc
-
Mus musculus Q9JJJ7 single gene and alternative splicing resulting in several variants
-
Xenopus laevis Q9I935 gene Xporc
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Drosophila melanogaster
-
embryo
-
Drosophila melanogaster
-
embryo
-
Mus musculus
-
fibroblast
-
Mus musculus
-
HEK-293 cell
-
Homo sapiens
-
additional information Mporc mRNA is differentially expressed during embryogenesis and in various adult tissues, demonstrating that the alternative splicing is regulated to synthesize the specific types of Mporc, pattern of expression of Mporc at fetal and adult stages of development, overview Mus musculus
-
oocyte
-
Xenopus laevis
-
S2 cell
-
Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(9Z)-hexadec-9-enoyl-CoA + [Wg]-L-serine
-
Drosophila melanogaster CoA + [Wg]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wg]-L-serine Wnt substrate from Drosophila melanogaster Mus musculus CoA + [Wg]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3]-L-serine
-
Drosophila melanogaster CoA + [Wnt3]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt5]-L-serine
-
Drosophila melanogaster CoA + [Wnt5]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine the enzyme catalyzes the palmitoylation of Wnt Wingless or Wg Drosophila melanogaster CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine the enzyme catalyzes the palmitoylation of Wnt Wingless or Wg. The enzyme binds the N-terminal 24-amino acid domain (residues 83-106) of Wg Drosophila melanogaster CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
-
Homo sapiens CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
-
Mus musculus CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
-
Drosophila melanogaster CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
-
Xenopus laevis CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
-
Caenorhabditis elegans CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
?
additional information enzyme Porc functions on the N-terminal 24-amino acid domain (83-106) of Wg, binding of Porc with Wg is essential for its activity Drosophila melanogaster ?
-
?
additional information recombinant c-Myc-tagged substrate Wg mutants T51A, S105A, S110A, and T416A Drosophila melanogaster ?
-
?

Synonyms

Synonyms Comment Organism
CG6205
-
Drosophila melanogaster
MG61
-
Homo sapiens
Mom-1
-
Caenorhabditis elegans
Mporc
-
Mus musculus
PORC
-
Drosophila melanogaster
PORC
-
Homo sapiens
PORCN
-
Homo sapiens
PORCN
-
Caenorhabditis elegans
PPN
-
Homo sapiens
Xporc
-
Xenopus laevis

General Information

General Information Comment Organism
evolution Drosophila Porc has an extra hydrophilic N-terminal sequence, which is not found in other Porc enzymes Drosophila melanogaster
additional information all Mporc types can substitute for Drosophila Porc, as they are able to rescue the phenotypes of Drosophila porc embryos, the enzyme modifies the processing of Drosophila Wg protein expressed in mammalian cells Mus musculus
additional information all Mus musculus Mporc types can substitute for Drosophila Porc, as they are able to rescue the phenotypes of Drosophila porc embryos Drosophila melanogaster
additional information model for the N-glycosylation of Wnt family, overview Drosophila melanogaster
physiological function ectopic expression of porc stimulates the N-glycosylation of both endogenously and exogenously expressed protein Wingless (Wg). The enzyme binds the N-terminal 24-amino acid domain (residues 83-106) of Wg, which is highly conserved in the Wnt family and stimulates the N-glycosylation at surrounding sites. The enzyme is also necessary for the processing of Drosophila Wnt-3/5 in both embryos and cultured cells. The enzyme binds the N-terminal specific domain of the Wnt family via its C-terminnus and stimulates its posttranslational N-glycosylation by anchoring them at the endoplasmic reticulum membrane possibly through acylation, overview Drosophila melanogaster
physiological function Mporc binds Wnt proteins and modifies their processing, the enzyme may function as a chaperone-like molecule for Wnt, overview Mus musculus
physiological function the enzyme is necessary for processing Wingless (Wg), a Drosophila Wnt (Wnt) family member. The enzyme modifies the processing of substrate Wg expressed in mammalian cells Drosophila melanogaster