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Literature summary for 2.3.1.247 extracted from

  • Barker, H.A.; Kahn, J.M.; Hedrick, L.
    Pathway of lysine degradation in Fusobacterium nucleatum (1982), J. Bacteriol., 152, 201-207.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.247

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
 Fusobacterium nucleatum subsp. nucleatum
EDTA activates at low concentrations in the presence of a higher concentration of CoCl2, inhibits at 0.1-1.0 mM by 30% Fusobacterium nucleatum subsp. nucleatum

Inhibitors

Inhibitors Comment Organism Structure
EDTA activates at low concentrations in the presence of a higher concentration of CoCl2, inhibits at 0.1-1.0 mM by 30% Fusobacterium nucleatum subsp. nucleatum
additional information no inhibition by DTNB Fusobacterium nucleatum subsp. nucleatum
potassium phosphate 4, 15, 30, 65, and 85% inhibition at 0.2, 0.4, 1.0, 10, and 100 mM, respectively Fusobacterium nucleatum subsp. nucleatum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ strong activation Fusobacterium nucleatum subsp. nucleatum
Mn2+ moderate activation Fusobacterium nucleatum subsp. nucleatum
additional information no activation by Mg2+ Fusobacterium nucleatum subsp. nucleatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(5S)-5-amino-3-oxohexanoate + acetyl-CoA Fusobacterium nucleatum subsp. nucleatum
-
 L-3-aminobutanoyl-CoA + acetoacetate
-
 r
(5S)-5-amino-3-oxohexanoate + acetyl-CoA Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847
-
 L-3-aminobutanoyl-CoA + acetoacetate
-
 r

Organism

Organism UniProt Comment Textmining
Fusobacterium nucleatum subsp. nucleatum Q8RHX2
-
-
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 Q8RHX2
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 143fold by anion exchange chromatography Fusobacterium nucleatum subsp. nucleatum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.18
-
 curde enzyme extract, pH 7.0, 25°C Fusobacterium nucleatum subsp. nucleatum
15.7
-
 purified enzyme, pH 7.0, 25°C Fusobacterium nucleatum subsp. nucleatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5S)-5-amino-3-oxohexanoate + acetyl-CoA
-
 Fusobacterium nucleatum subsp. nucleatum L-3-aminobutanoyl-CoA + acetoacetate
-
 r
(5S)-5-amino-3-oxohexanoate + acetyl-CoA
-
 Fusobacterium nucleatum subsp. nucleatum L-3-aminobutanoyl-CoA + acetoacetate DL-3-aminobutanoyl-CoA and acetoacetate are used in the reverse reaction assay r
(5S)-5-amino-3-oxohexanoate + acetyl-CoA
-
 Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 L-3-aminobutanoyl-CoA + acetoacetate
-
 r
(5S)-5-amino-3-oxohexanoate + acetyl-CoA
-
 Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 L-3-aminobutanoyl-CoA + acetoacetate DL-3-aminobutanoyl-CoA and acetoacetate are used in the reverse reaction assay r
beta-alanyl-CoA + acetoacetate
-
 Fusobacterium nucleatum subsp. nucleatum ?
-
 r
beta-alanyl-CoA + acetoacetate
-
 Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 ?
-
 r
DL-3-aminobutanoyl-CoA + 2-methylacetoacetate
-
 Fusobacterium nucleatum subsp. nucleatum (5S)-5-amino-2-methyl-3-oxohexanoate + acetyl-CoA
-
 r
DL-3-aminobutanoyl-CoA + 2-methylacetoacetate
-
 Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 (5S)-5-amino-2-methyl-3-oxohexanoate + acetyl-CoA
-
 r

Synonyms

Synonyms Comment Organism
3-keto,5-aminohexanoate cleavage enzyme
-
 Fusobacterium nucleatum subsp. nucleatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 forward reaction Fusobacterium nucleatum subsp. nucleatum

pH Range

pH Minimum pH Maximum Comment Organism
5.2 8.1 50% of maximal activity at pH 5.2 and pH 8.1 Fusobacterium nucleatum subsp. nucleatum

General Information

General Information Comment Organism
metabolism the enzyme is involved in the the 3-keto-5-aminohexanoate pathway of lysine degradation, tracer experiments on acetate and butyrate formation from lysine and acetate, overview Fusobacterium nucleatum subsp. nucleatum