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Literature summary for 2.3.1.240 extracted from

  • Lu, H.; Tsai, S.; Khosla, C.; Cane, D.
    Expression, site-directed mutagenesis, and steady state kinetic analysis of the terminal thioesterase domain of the methymycin/picromycin polyketide synthase (2002), Biochemistry, 41, 12590-12597.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the thioesterase domain of the methymycin/picromycin synthase (PICS) is functionally expressed in Escherichia coli Streptomyces venezuelae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady state kinetic parameters for diketides with wild-type and mutant thioesterase domain of methymycin/picromycin synthase Streptomyces venezuelae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + 6 (2S)-methylmalonyl-CoA + 5 NADPH + 5 H+ Streptomyces venezuelae the product narbonolide is an intermediate in the biosynthesis of methymycin narbonolide + 7 CoA + 7 CO2 + 5 NADP+ + 2 H2O
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Organism

Organism UniProt Comment Textmining
Streptomyces venezuelae Q9ZGI5 and Q9ZGI4 and Q9ZGI3 and Q9ZGI2 and Q9ZGI1 Q9ZGI5: type I polyketide synthase PikAI, Q9ZGI4: type I polyketide synthase PikAII, Q9ZGI3: type I polyketide synthase PikAIII, Q9ZGI2: type I polyketide synthase PikAIV, Q9ZGI1: thioesterase II PikAV
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + 6 (2S)-methylmalonyl-CoA + 5 NADPH + 5 H+ the product narbonolide is an intermediate in the biosynthesis of methymycin Streptomyces venezuelae narbonolide + 7 CoA + 7 CO2 + 5 NADP+ + 2 H2O
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malonyl-CoA + 6 (2S)-methylmalonyl-CoA + 5 NADPH + 5 H+ the enzyme also produces 10-deoxymethynolide (see EC 2.3.1.239, 10-deoxymethynolide synthase). The enzyme has 29 active sites arranged in four polypeptides (pikAI - pikAIV) with a loading domain, six extension modules and a terminal thioesterase domain. Each extension module contains a ketosynthase (KS), keto reductase (KR), an acyltransferase (AT) and an acyl-carrier protein (ACP). Not all active sites are used in the biosynthesis Streptomyces venezuelae narbonolide + 7 CoA + 7 CO2 + 5 NADP+ + 2 H2O
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Synonyms

Synonyms Comment Organism
methymycin/picromycin polyketide synthase
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Streptomyces venezuelae
PICS
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Streptomyces venezuelae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information steady state kinetic parameters for diketides with wild-type and mutant thioesterase domain of methymycin/picromycin synthase Streptomyces venezuelae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pH-dependence of the hydrolysis of thioesters by the thioesterase domain of methymycin/picromycin synthase with diketides Streptomyces venezuelae

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthesis of narbonomycin Streptomyces venezuelae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
additional information steady state kinetic parameters for diketides with wild-type and mutant thioesterase domain of methymycin/picromycin synthase Streptomyces venezuelae