Cloned (Comment) | Organism |
---|---|
the thioesterase domain of the methymycin/picromycin synthase (PICS) is functionally expressed in Escherichia coli | Streptomyces venezuelae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady state kinetic parameters for diketides with wild-type and mutant thioesterase domain of methymycin/picromycin synthase | Streptomyces venezuelae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + 6 (2S)-methylmalonyl-CoA + 5 NADPH + 5 H+ | Streptomyces venezuelae | the product narbonolide is an intermediate in the biosynthesis of methymycin | narbonolide + 7 CoA + 7 CO2 + 5 NADP+ + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces venezuelae | Q9ZGI5 and Q9ZGI4 and Q9ZGI3 and Q9ZGI2 and Q9ZGI1 | Q9ZGI5: type I polyketide synthase PikAI, Q9ZGI4: type I polyketide synthase PikAII, Q9ZGI3: type I polyketide synthase PikAIII, Q9ZGI2: type I polyketide synthase PikAIV, Q9ZGI1: thioesterase II PikAV | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + 6 (2S)-methylmalonyl-CoA + 5 NADPH + 5 H+ | the product narbonolide is an intermediate in the biosynthesis of methymycin | Streptomyces venezuelae | narbonolide + 7 CoA + 7 CO2 + 5 NADP+ + 2 H2O | - |
? | |
malonyl-CoA + 6 (2S)-methylmalonyl-CoA + 5 NADPH + 5 H+ | the enzyme also produces 10-deoxymethynolide (see EC 2.3.1.239, 10-deoxymethynolide synthase). The enzyme has 29 active sites arranged in four polypeptides (pikAI - pikAIV) with a loading domain, six extension modules and a terminal thioesterase domain. Each extension module contains a ketosynthase (KS), keto reductase (KR), an acyltransferase (AT) and an acyl-carrier protein (ACP). Not all active sites are used in the biosynthesis | Streptomyces venezuelae | narbonolide + 7 CoA + 7 CO2 + 5 NADP+ + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methymycin/picromycin polyketide synthase | - |
Streptomyces venezuelae |
PICS | - |
Streptomyces venezuelae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady state kinetic parameters for diketides with wild-type and mutant thioesterase domain of methymycin/picromycin synthase | Streptomyces venezuelae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-dependence of the hydrolysis of thioesters by the thioesterase domain of methymycin/picromycin synthase with diketides | Streptomyces venezuelae |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthesis of narbonomycin | Streptomyces venezuelae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady state kinetic parameters for diketides with wild-type and mutant thioesterase domain of methymycin/picromycin synthase | Streptomyces venezuelae |