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Literature summary for 2.3.1.239 extracted from

  • Chemler, J.A.; Tripathi, A.; Hansen, D.A.; ONeil-Johnson, M.; Williams, R.B.; Starks, C.; Park, S.R.; Sherman, D.H.
    Evolution of efficient modular polyketide synthases by homologous recombination (2015), J. Am. Chem. Soc., 137, 10603-10609.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis versatile method for generating and identifying functional chimeric PKS enzymes for synthesizing custom macrolactones and macrolides. PKS genes from the pikromycin and erythromycin pathways are hybridized in Saccharomyces cerevisiae to generate hybrid libraries. Streptomyces venezuelae strains that expressed active chimeric enzymes with new functionality are capable of producing engineered macrolactones Streptomyces venezuelae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Streptomyces venezuelae Streptomyces venezuelae

Organism

Organism UniProt Comment Textmining
Streptomyces venezuelae
-
-
-
Streptomyces venezuelae ATCC 15439
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methylmalonyl-N-acetylcysteamine + S-phenyl (2S,4R,6E,8R,9R)-9-hydroxy-2,4,8-trimethyl-5-oxoundec-6-enethioate
-
Streptomyces venezuelae 3-dehydro-10-deoxymethynolide + 10-deoxymethynolide + CoA + CO2 + NADP+ + H2O products formed by chimeric polyketide synthase construcuts ?
methylmalonyl-N-acetylcysteamine + S-phenyl (2S,4R,6E,8R,9R)-9-hydroxy-2,4,8-trimethyl-5-oxoundec-6-enethioate
-
Streptomyces venezuelae ATCC 15439 3-dehydro-10-deoxymethynolide + 10-deoxymethynolide + CoA + CO2 + NADP+ + H2O products formed by chimeric polyketide synthase construcuts ?

Synonyms

Synonyms Comment Organism
PikAIII
-
Streptomyces venezuelae
PikAIV
-
Streptomyces venezuelae

Cofactor

Cofactor Comment Organism Structure
additional information enzyme utilizes an oxidized quinone as co-factor Streptomyces venezuelae