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Literature summary for 2.3.1.233 extracted from

  • Meslet-Cladiere, L.; Delage, L.; Leroux, C.J.; Goulitquer, S.; Leblanc, C.; Creis, E.; Gall, E.A.; Stiger-Pouvreau, V.; Czjzek, M.; Potin, P.
    Structure/function analysis of a type III polyketide synthase in the brown alga Ectocarpus siliculosus reveals a biochemical pathway in phlorotannin monomer biosynthesis (2013), Plant Cell, 25, 3089-3103.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.233

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) codon Plus RIPL cells Ectocarpus siliculosus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M Bicine, pH 8, and 18% (w/v) polyethylene glycol 6000, at 19°C Ectocarpus siliculosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA Ectocarpus siliculosus
-
 1,3,6,8-tetrahydroxynaphthalene + CoA + CO2 + H2O
-
 ?
malonyl-CoA Ectocarpus siliculosus Ec 32
-
 1,3,6,8-tetrahydroxynaphthalene + CoA + CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Ectocarpus siliculosus D8LJ35
-
-
Ectocarpus siliculosus Ec 32 D8LJ35
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-nickel tetradentate column chromatography and Sephacryl-200 gel filtration Ectocarpus siliculosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Acetyl-CoA
-
 Ectocarpus siliculosus ?
-
 ?
Acetyl-CoA
-
 Ectocarpus siliculosus Ec 32 ?
-
 ?
lauroyl-CoA
-
 Ectocarpus siliculosus 1-(2,4,6-trihydroxyphenyl)-dodecan-1-one + 4-hydroxy-6-(2-oxotridecyl)-2H-pyran-2-one + CoA + CO2 + H2O
-
 ?
lauroyl-CoA
-
 Ectocarpus siliculosus Ec 32 1-(2,4,6-trihydroxyphenyl)-dodecan-1-one + 4-hydroxy-6-(2-oxotridecyl)-2H-pyran-2-one + CoA + CO2 + H2O
-
 ?
malonyl-CoA
-
 Ectocarpus siliculosus 1,3,6,8-tetrahydroxynaphthalene + CoA + CO2 + H2O
-
 ?
malonyl-CoA
-
 Ectocarpus siliculosus Ec 32 1,3,6,8-tetrahydroxynaphthalene + CoA + CO2 + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Ectocarpus siliculosus

Synonyms

Synonyms Comment Organism
PhlD
-
 Ectocarpus siliculosus
PKS III
-
 Ectocarpus siliculosus
PKS1
-
 Ectocarpus siliculosus
type III polyketide synthase
-
 Ectocarpus siliculosus

General Information

General Information Comment Organism
metabolism the enzyme catalyzes a major step in the biosynthetic pathway of phlorotannins (i.e., the synthesis of phloroglucinol monomers from malonyl-CoA) Ectocarpus siliculosus